@misc{oai:repo.qst.go.jp:00071462,
 author = {Desai, Sejal and Kobayashi, Alisa and Konishi, Teruaki and Oikawa, Masakazu and N., Pandey Badri and 小林 亜利紗 and 小西 輝昭 and 及川 将一},
 month = {Mar},
 note = {We reports on radiation induced bystander effect with relevance to cancer radiotherapy especially investigating the bidirectional and criss-cross bystander communications between cancer and normal cells are limited. In this study employing co-culture approach, we have investigated the bystander cross-talk between A549 lung cancer and WI38 normal cells after proton-microbeam irradiation using γ-H2AX foci fluorescence as a measure of DNA double-strand breaks (DSBs). We observed that in A549-A549 co-cultures, irradiated A549 cells exert damaging effects in bystander A549 cells, which were found to be mediated through gap junctional intercellular communication (GJIC). However, in A549-WI38 co-cultures irradiated A549 did not affect bystander WI38 cells. Rather, bystander WI38 cells induced inverse protective signaling (rescue effect) in irradiated A549 cells, which was independent of GJIC. On the other hand, in response to irradiated WI38 cells neither of the bystander cells (A549 or WI38) showed significant increase in γ-H2AX foci. The observed bystander signaling between tumor and normal cells may have potential implications in therapeutic outcome of cancer radiotherapy., 第４回共用施設（PASTA&SPICE, NASBEE)共同研究成果報告会},
 title = {Damaging and protective bystander cross-talk between human lung cancer and normal cells after proton microbeam irradiation.},
 year = {2014}
}