@misc{oai:repo.qst.go.jp:00071312,
 author = {Sato, Katsutoshi and Irie, Daisuke and Nakawatari, Miyako and Nakamura, Etsuko and Moritake, Hiroyuki and Imai, Takashi and Shimokawa, Takashi and 佐藤 克俊 and 入江 大介 and 中渡 美也子 and 中村 悦子 and 森竹 浩之 and 今井 高志 and 下川 卓志},
 month = {May},
 note = {The aim of this study is to establish carbon ion beam (C-ion) resistant cancer cells and to investigate the difference in cellular characteristics between C-ion resistant and X-ray resistant cancer cells. The X-ray resistant cancer cell was already established using mouse squamous cell carcinoma cell line NRS1. The cells were repeatedly irradiated with a total dose 60 Gy of 200 kVp X-ray, and this repetitive X-ray irradiation generated the X-ray resistant cancer cell NRS1-X60. Surprisingly the result from colony formation assay after irradiation of 290 MeV/n C-ion showed that NRS1-X60 cells also acquired significant C-ion resistance, Although C-ion irradiation is known to be more effective for killing cells of X-ray resistant tumor. The survival fractions of NRS1-X60 cells at 10 Gy of X-ray or 5 Gy of C-ion is approximately 6.3- or 6.7-folds increased compared with that in NRS1 cells, respectively. DNA repair potential in NRS1-X60 cells, which is represented by the disappearance of g-H2AX foci at one day after X-ray or C-ion irradiation, is promoted by approximately 2.3-fold compared with that in NRS1 cells for both irradiations. Currently, the NRS1 cells were repeatedly irradiated with a total dose 30 Gy of C-ion, and we established NRS1-C30 cells. We will report the difference in X-ray, C-ion sensitivity, and DNA repair potential between NRS1-X60, NRS1-C30, and NRS1., Heavy Ion in Therapy and Space Radiation Symposium 2013 (HITSRS2013)},
 title = {Establishment and characterization of novel carbon-ion beam resistance cells},
 year = {2013}
}