@misc{oai:repo.qst.go.jp:00071285,
 author = {Shimokawa, Takashi and Fujita, Hidetoshi and Hosoi, Akihiro and Sato, Katsutoshi and Kakimi, Kazuhiro and Imai, Takashi and 下川 卓志 and 藤田 英俊 and 佐藤 克俊 and 今井 高志},
 month = {Oct},
 note = {Carbon-ion (C-ion) radiotherapy (RT) is an advanced RT effective in tumors that are resistant to conventional RT due to its biological properties and excellent dose distribution. However, metastasis control is an important issue to improve C-ion RT. 
We previously reported that the combination of C-ion irradiation with dendritic cell (DC) immunotherapy significantly suppressed lung metastases in murine models, such as NRS1 mouse squamous cell carcinoma cell transplanted C3H/He mouse. However, the underlying mechanisms are still not clearly understood. In this study, we aim to clarify the impact of C-ion irradiation on the combination therapy in terms of immature DC (iDC) activation. To investigate the effect of C-ion irradiation on DC immunotherapy, we examined whether irradiated or non-irradiated cancer cells can activate immature DC cells by co-culture. NRS1 cells were irradiated with C-ion, then co-cultured for three days with iDCs, which were isolated from C3H/He mice. Activation of the DC was evaluated by a flow cytometer and real-time qPCR. It clearly showed that iDCs were effectively activated by C-ion irradiated cancer cells, but not by non-irradiated cancer cells., The 72nd Annual Meeting of the Japanese Cancer Association},
 title = {Analysis of underlying mechanisms for combination therapy of carbon-ion irradiation and dendritic cell immunotherapy.},
 year = {2013}
}