@misc{oai:repo.qst.go.jp:00071164,
 author = {Jin, Zhao-Hui and Furukawa, Takako and Sogawa, Chizuru and Tsuji, Atsushi and U, Winn Aung and Coll, Jean-Luc and Saga, Tsuneo and et.al and 金 朝暉 and 古川 高子 and 曽川 千鶴 and 辻 厚至 and Ｕ Ｗｉｎｎ Ａｕｎｇ and 佐賀 恒夫},
 month = {Jun},
 note = {Objective: 64Cu-cyclam-RAFT-c(-RGDfK-)4 (64Cu-RAFTRGD) is a novel tetrameric cyclic RGD peptide probe for PET imaging of tumor angiogenesis via targeting the V3 integrin. Because 64Cu also emits -, it can be used for internal radiotherapy. We studied the effects of a succinylated gelatin-containing plasma expander Gelofusine and L-lysine on the renal uptake and retention of 64Cu-RAFTRGD in tumor-bearing mice, aiming to find measures to lower the risk of nephrotoxicity in internal radiotherapy using 64Cu-RAFTRGD.
Methods: Normal or tumor-bearing mice received injection of 64Cu-RAFTRGD with or without co-injection of Gelofusine, L-lysine, or the both. Biodistribution studies were performed at 3 and 24 h post-injection (p.i.). Dynamic PET scans (5 min/frame) were performed at 0-60 min p.i., followed by static PET scans at 3.5 and 24 h p.i. Radio-TLC was done to analyze the radioactive metabolites in blood, urine, liver, and kidney samples at 1 and 24 h p.i. 
Results: Co-injection of Gelofusine significantly reduced the renal uptake and retention of 64Cu-RAFTRGD. Although L-lysine alone had no influence on the renal radioactivity accumulation, it showed the tendency to enhance the inhibitory effect of Gelofusine. The uptake of 64Cu-RAFTRGD in the tumors was not influenced by co-injection of Gelofusine or together with L-lysine. Dynamic PET imaging of mice and metabolic analysis of tissue samples clearly showed that Gelofusine did block the renal reabsorption of 64Cu-RAFTRGD, and did not interfere with the metabolism and excretion pathway of the probe.
Conclusion: Administration of Gelofusine or together with L-lysine is a promising measure for kidney protection in internal radiotherapy using 64Cu-RAFTRGD., SNMMI 2013 Annual Meeting},
 title = {PET imaging and quantification of the effects of succinylated gelatin and/or L-lysine on renal uptake and retention of the novel radiopharmaceutical 64Cu-cyclam-RAFT-c(-RGDfK-)4},
 year = {2013}
}