@misc{oai:repo.qst.go.jp:00071106,
 author = {Vares, Guillaume and Nakajima, Tetsuo and Bing, Wang and Ishii, Hiroko and Nenoi, Mitsuru and Ｇｕｉｌｌａｕｍｅ Ｖａｒｅｓ and 中島 徹夫 and 王 冰 and 石井 洋子 and 根井 充},
 month = {May},
 note = {Obesity has been associated with various pathologies including cancer. It is estimated that up to 7% of all new cancers in US were due to obesity. Although some relationships were described between diet and ionizing radiation, there is still much to know about the dietary influences on radiation risks. In order to evaluate the modulation of radiation responses according to calorie intake, we exposed C3H and C57BL/6 mice fed a normal diet or a high-fat(HF) diet to fractionated doses of X-rays. Bone marrow micronucleus assays suggested that HF diet did not modulate radiation-induced genotoxicity. Both HF diet and irradiation rescued in increased oxidative damage, H2AX levels and proliferation in C57BL/6 mouse liver sections. Using methylation-specific PCR, we observed that the promoters of p16 and Dapk gene were methylated only in the livers of C57BL/6 mice fed a HF diet (irradiated and non-irradiated); Mgmt promoter was methylated in irradiated and/or HF diet-fed mice. In addition, methylation PCR arrays identified Ep300 and Socs1 (whose promoters exhibited higher methylation levels in non-irradiated HF diet-fed mice) as potential target for further studies. We then compared microRNA regulations after radiation exposure in the livers of C57BL/6mice fed a normal or an HF diet, using microRNA arrays. Interestingly, radiation-specific microRNA regulations observed in normal mice were not observed in obese mice, fed a HF diet. All together, our results suggested the existence of dietary effects on radiation responses in mice., The 3rd Asian Congress of Radiation Research},
 title = {High-fat diet induces changes in DNA methylation and microRNA regulations after exposure to ionizing radiation},
 year = {2013}
}