{"created":"2023-05-15T14:51:59.006938+00:00","id":71061,"links":{},"metadata":{"_buckets":{"deposit":"ecdf23eb-c6f2-47ad-bb51-bca84136d61b"},"_deposit":{"created_by":1,"id":"71061","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"71061"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00071061","sets":["10:28"]},"author_link":["698462","698466","698463","698465","698464","698461"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-02-25","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We have previously reported that Carbon ion (C-ion) irradiation suppresses the invasiveness of several pancreatic cancer cell lines, MIAPaCa-2, BxPC-3, and AsPC-1 cells, however, we also observed that C-ion irradiation enhanced invasion in PANC-1 cells, which occurred via the activation of serine proteases (SerP), plasmin, and uPA. Treatment of C-ion irradiated PANC-1 with SerP inhibitor and ROCK inhibitor resulted in reduced invasiveness, but only by 50%, suggesting that there are more factors that have a role in C-ion-irradiation-induced PANC-1 invasion. To study the molecular factors involved in irradiation-induced PANC-1 invasion, we used the Screening Committee of Anticancer Drugs (SCADS) compound library and found that nitric oxide synthase (NOS) inhibitors and phosphatidylinositol 3-kinases (PI3K) inhibitors were both effective in reducing PANC-1 invasion. Most invaded PANC-1 cells were nitric oxide (NO)-producing cells, and the NOS-PI3K-AKT pathway was activated in these cells. This effect could be abrogated by NOS inhibitor treatment, which also reduced invasion. The population of NO-producing cells was increased by C-ion irradiation, thereby enhancing C-ion-irradiation-induced PANC-1 invasion. These results suggested that irradiation alters the invasive potential through NO and NOS-PI3K-AKT pathway activities in addition to the SerP activity.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"Ninth AACR-Japanese Cancer Association Joint Conference","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fujita, Mayumi"}],"nameIdentifiers":[{"nameIdentifier":"698461","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamada, Shigeru"}],"nameIdentifiers":[{"nameIdentifier":"698462","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imai, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"698463","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤田 真由美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"698464","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田 滋","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"698465","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井 高志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"698466","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Irradiation alters the invasive potential of PANC-1 cell line thorough NO and NOS-PI3K-AKT pathway activities in addition to the SerP activity","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Irradiation alters the invasive potential of PANC-1 cell line thorough NO and NOS-PI3K-AKT pathway activities in addition to the SerP activity"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-03-08"},"publish_date":"2013-03-08","publish_status":"0","recid":"71061","relation_version_is_last":true,"title":["Irradiation alters the invasive potential of PANC-1 cell line thorough NO and NOS-PI3K-AKT pathway activities in addition to the SerP activity"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:55:39.262606+00:00"}