@misc{oai:repo.qst.go.jp:00071005,
 author = {Sogawa, Chizuru and Tsukamoto, Satoshi and Ishida, Yuka and Yoshii, Yukie and Furukawa, Takako and KUNIEDA, Tetsuo and Saga, Tsuneo and et.al and 曽川 千鶴 and 塚本 智史 and 石田 有香 and 吉井 幸恵 and 古川 高子 and 佐賀 恒夫},
 month = {Jul},
 note = {C-type natriuretic peptide (CNP) exerts its main biological effects by binding to natriuretic peptide receptor B (NPR-B), a membrane-bound guanylyl cyclase receptor that produces cyclic guanosine monophosphate (cGMP). It was previously reported that cGMP induced by nitric oxide via soluble guanylyl cyclase regulates Sertoli cell tight junction in vitro and permeability of the blood-testis barrier (BTB) which separates the seminiferous epithelium into the adluminal and basal compartments during spermatogenesis. Furthermore, it was also reported that CNP regulates BTB dynamics in adult rat testes. 
 We recently reported that the short-limbed dwarfism (SLW) mouse has a mutation in Npr-2 gene, which encodes NPR-B and this mutation results in deletion of the intracellular domain of NPR-B. However, the effect of the flaw in the NPR-B-induced cGMP production through CNP signal against spermatogenesis has not yet been demonstrated. Therefore, the aim of this study is to elucidate the function of NPR-B in the progression of spermatogenesis in mouse.
 We first examined the immunohistochemical distribution of NPR-B in the seminiferous epithelium of the normal mouse testis during the first-wave of spermatogenesis. NPR-B was initially expressed within a spermatogonium, and subsequently located to the cytoplasm of a spermatocyte and a round spermatid predominantly in the normal testes. We next examined the phenotype of testes in SLW mice. It was revealed that the testis of slw/slw homozygotes showed retardation of spermatogenic development in 2w and vacuolation in the seminiferous epithelium in 3w. Immunohistochemical staining of ZO-1, a tight junction protein revealed that BTB was located in a boundary between the basement and the lumen in the seminiferous epithelium of 2w normal testis, whereas BTB formation was disordered and misaligned in the 2w slw/slw.
 These results suggest that NPR-B mediated cGMP production through CNP signal has a role important for BTB regulation which is required for spermatogenesis., 58th/60th NIBB Conferenc},
 title = {CNP Receptor Gene (Npr2) is Involved in the Regulation of Blood-Testis Barrier and Spermatogenesis in Mouse},
 year = {2012}
}