{"created":"2023-05-15T14:51:52.894610+00:00","id":70922,"links":{},"metadata":{"_buckets":{"deposit":"7d7b9b3d-b2ad-4415-92f6-76dd9e979124"},"_deposit":{"created_by":1,"id":"70922","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70922"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070922","sets":["10:28"]},"author_link":["696880","696878","696879","696881"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-09-27","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The long-lived B-emitter technetium-99 (Tc-99) is a man-made fission product of U-235 and Pu-239 with a relatively high fission yield of about 6%.  In aerobic environments, Tc is normally present in a heptavalent form as pertechnetate (TcO4-).  It has been thought that phytoplankton is unsuitable for remediation of Tc-99 contaminated waters.  In this study, removal of Tc-99 by freshwater phytoplankton Euglena gracilis was described.\nE. gracilis was cultured in a solution containing Tc-99 for 63 days at 20C under 12 h light-dark cycles.  Removal of Tc-99 from the solution was periodically determined using a Tri-Carb 2500TR liquid scintillation analyzer.  Spirulina platensis (NIES-39), Chlamydomonas pulsatilla (NIES-122) and Chlorella vulgaris (NIES-227) were also tested for the removal of Tc-99.\nAmong phytoplankton used, only E. gracilis removed significant amounts of Tc-99 from the solution as they grew.  The removal continued for 50 days, at which time equilibrium was achieved.  The maximum value of the removal was 70% of the total Tc-99 added.  To clarify the intracellular uptake of Tc-99 by E. gracili, the removal of Tc-99 by killed cells was carried out.  E. gracilis cells were killed by the addition of a protein synthesis inhibitor cycloheximid at a final concentration of 50 ug mL-1 or by heating cells at 60C for 1 hour.  In both cultures, E. gracilis removed negligible amounts of Tc-99.  In addition to the killed cells experiment, desorption of Tc-99 from cell surface were carried out.  As the pH of the solution was increased from 3.5 to 7.5, a slight increase in desorption amounts of the Tc-99 was observed.  The relative amount of desorption, however, was, at a maximum, 14% of the total Tc-99 removed.  These results suggest that a slight amount of Tc-99 had been adsorbed onto the cell surface.  Results of both the killed cell experiments and desorption experiments show that the removal of Tc-99 by E. gracilis was primarily the result of intracellular uptake.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"SETAC Asia Pacific 2012 Meeting","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ishii, Nobuyoshi"}],"nameIdentifiers":[{"nameIdentifier":"696878","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Uchida, Shigeo"}],"nameIdentifiers":[{"nameIdentifier":"696879","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石井 伸昌","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"696880","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"内田 滋夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"696881","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Removal of technetium-99 by Euglena gracilis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Removal of technetium-99 by Euglena gracilis"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-10-02"},"publish_date":"2012-10-02","publish_status":"0","recid":"70922","relation_version_is_last":true,"title":["Removal of technetium-99 by Euglena gracilis"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:57:16.555521+00:00"}