{"created":"2023-05-15T14:51:52.590586+00:00","id":70915,"links":{},"metadata":{"_buckets":{"deposit":"8b51b42c-8fb1-4958-a0e7-8a565c1b11ed"},"_deposit":{"created_by":1,"id":"70915","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70915"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070915","sets":["10:28"]},"author_link":["696827","696824","696828","696829","696830","696835","696833","696834","696826","696832","696831","696825"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-09-21","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: The mechanisms of regional hemodynamic response during neural activation including the concept of neurovascular coupling have been investigated using anesthetized rodent models. However, anesthesia significantly affects the physiological state including regulation of cerebral circulation throughout the brain. The purpose of this study was to explore changes in hemodynamic characteristics during sensory stimulation in awake animals as compared with those in anesthetized animals. Methods: A total of 18 male C57BL/6J mice (20-30 g, 7-11 weeks) were used in this study. Changes in CBF, red blood cell (RBC) velocity and concentration in the somatosensory cortex during whisker stimulation were measured using laser-Doppler flowmetry in awake and anesthetized mice. Results: The degree of increase in RBC velocity during whisker stimulation was far higher than that of the increase in the RBC concentration under the awake condition (increase in RBC velocity: 18.4+7.5 %, RBC concentration: 1.7+2.9 %). 1.5% Isoflurane-induced anesthesia attenuated the increase in RBC velocity and concentration during stimulation (increase in RBC velocity: 103.4+2.9%, RBC concentration: 1.4+1.0%), and the attenuation of increase in RBC velocity was prominent as compared to the change in RBC concentration. During the resting condition, significant differences in baseline CBF, RBC velocity and concentration were not observed between awake and anesthesia mice. Conclusion: Isoflurane-induced anesthesia attenuated the increase in CBF during stimulation that was mainly caursed by increase in RBC velocity rather than RBC concentration, indicating the decreased energy demand in the brain as a result of the attenuation of neuronal activity due to anesthesia might inhibit the increase in CBF during stimulation. The RBC measurement techniques in awake animals are likely useful for the investigation of the hemodynamic changes caused by changes in neural activity or other chemical factors like PaCO2.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"The 35th annual meeting of the japan neuroscience society","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takuwa, Hiroyuki"}],"nameIdentifiers":[{"nameIdentifier":"696824","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsuura, Tetsuya"}],"nameIdentifiers":[{"nameIdentifier":"696825","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Obata, Takayuki"}],"nameIdentifiers":[{"nameIdentifier":"696826","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawaguchi, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"696827","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kanno, Iwao"}],"nameIdentifiers":[{"nameIdentifier":"696828","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ito, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"696829","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田桑 弘之","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"696830","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松浦 哲也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"696831","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小畠 隆行","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"696832","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"川口 拓之","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"696833","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"菅野 巖","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"696834","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伊藤 浩","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"696835","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Changes in RBC velocity and concentration during whisker stimulation in awake and anesthetized mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Changes in RBC velocity and concentration during whisker stimulation in awake and anesthetized mice"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-09-26"},"publish_date":"2012-09-26","publish_status":"0","recid":"70915","relation_version_is_last":true,"title":["Changes in RBC velocity and concentration during whisker stimulation in awake and anesthetized mice"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:57:21.385168+00:00"}