@misc{oai:repo.qst.go.jp:00070744,
 author = {Fujita, Hidetoshi and Ando, Ken and Nakamura, Etsuko and Nakawatari, Miyako and Imai, Takashi and Shimokawa, Takashi and 藤田 英俊 and 安藤 謙 and 中村 悦子 and 中渡 美也子 and 今井 高志 and 下川 卓志},
 month = {May},
 note = {[Objectives] The carbon-ion (C-ion) radiotherapy (RT) is an advanced RT using positively-charged carbon-ion particles. Due to the biological properties and excellent dose distribution, the C-ion RT is effective in resistance tumor to the conventional RT. However, metastasis control is one of the important issues in C-ion RT. Therefore, we have examined the possibility of control of metastasis by combined therapy with C-ion. In this study, we focused on a combined therapy of C-ion irradiation and dendritic cell (DC) immunotherapy and examined the effects of this treatment in the mouse lung metastatic models.
[Methods] The mouse carcinoma cell lines (NR-S1, LLC, LM8) inoculated into the legs of C3H/He or C57BL/6J mice were irradiated with a single dosage of carbon-ion (6Gy or 2 Gy, 290 MeV/nucleon, 6 cm SOBP). At 36 hours after irradiation, DCs were injected into the mice. The numbers of lung metastasis were evaluated using
pathological observations.
[Results] We established the mouse metastasis model using squamous carcinoma cell line NR-S1, lung cancer cell line, LLC, and osteosarcoma cell line, LM8. Under conditions that there are no significant effects by the treatments on the growth of transplanted tumors, the number of lung metastasis was significantly decreased
by the combined therapy.
[Conclusions] Our present results demonstrated that combined therapy of C-ion irradiation and DC immunotherapy was effective in repression of lung metastasis against these tumors., The 2nd Japan-China Symposium on Cancer Research},
 title = {A combination therapy of carbon-ion irradiation and dendritic cell immunotherapy in mouse lung metastatic models.},
 year = {2012}
}