{"created":"2023-05-15T14:51:40.399996+00:00","id":70637,"links":{},"metadata":{"_buckets":{"deposit":"45f521b3-b0a6-4a5c-b884-ff9b452d7b6e"},"_deposit":{"created_by":1,"id":"70637","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70637"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070637","sets":["10:28"]},"author_link":["693828","693830","693829","693827"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2011-11-20","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Hydroxyl radical (OH) is one of the most important players in oxidative stress. The OH can be measured using electron paramagnetic resonance (EPR) spin-trapping method. Generally, OH-scavenging-effect of an anti-oxidant has been estimated by a method adding the anti-oxidant and a spin-trapping agent to a OH generation system. X-ray irradiation to water can generate OH. In this simple system, estimation of the OH-scavenging-effect of an anti-oxidant can be much easier, while reduction of spin adduct with the anti-oxidant must be considered. In this study, we are trying to propose an efficient method for estimating OH-scavenging-effect of an anti-oxidant.\nA reaction mixture containing 30 mM of a spin-trapping agent (DMPO) and an arbitrary concentration of an anti-oxidant was prepared using 100 mM phosphate buffer (pH7, containing 0.05 mM DTPA). X-ray was irradiated to the reaction mixture and then time course of the OH-spin-adduct (DMPO-OH) was measured immediately using X-band EPR spectrometer.\nX-ray irradiation to the reaction mixture containing DMPO generates DMPO-OH in the reaction mixture dose dependently. Even doing the experiment under N2 gas atmosphere, no difference was obtained for amount of DMPO-OH generated compared to the experiment in the air. The DMPO-OH obtained in the reaction mixture was from OH generated by X-ray. When anti-oxidant type 1 was added to the reaction mixture, amount of X-ray induced DMPO-OH in the reaction mixture was decreased depending on the dose of the anti-oxidant type 1. To determine whether the anti-oxidant type 1 was really scavenging OH, the reduction rate of DMPO-OH after irradiation was estimated. The reduction rate of DMPO-OH by the anti-oxidant type 1 was enough slow; therefore, the reducing DMPO-OH amount by adding anti-oxidant before X-ray irradiation was mainly due to scavenging OH by the anti-oxidant type 1. Anti-oxidant type 2 also showed the reducing DMPO-OH amount, when the anti-oxidant type 2 was added to reaction mixture before X-ray irradiation. Anti-oxidant type 2, however, showed a rapid reduction of DMPO-OH after stopping X-ray irradiation. Scavenging OH by anti-oxidant 2 can not estimated directly from the result of the experiment; however, it is possible to think that anti-oxidant 2 was a strong reductant which can be reduce relatively stable nitroxyl radical (DMPO-OH in this case). Using the method in this experiment, anti-oxidative property can be estimated using scavenging OH and/or reducing DMPO-OH.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"18th Annual Meeting of the Society for Free Radical Biology and Medicine","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Matsumoto, Kenichiro"}],"nameIdentifiers":[{"nameIdentifier":"693827","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakanishi, Ikuo"}],"nameIdentifiers":[{"nameIdentifier":"693828","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本 謙一郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"693829","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中西 郁夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"693830","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Efficient Method for Hydroxyl Radical Scavenging Activity of Antioxidants","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Efficient Method for Hydroxyl Radical Scavenging Activity of Antioxidants"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-11-24"},"publish_date":"2011-11-24","publish_status":"0","recid":"70637","relation_version_is_last":true,"title":["Efficient Method for Hydroxyl Radical Scavenging Activity of Antioxidants"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:00:37.518000+00:00"}