@misc{oai:repo.qst.go.jp:00070576,
 author = {Matsumoto, Yoshitaka and Uzawa, Akiko and Hirayama, Ryoichi and Koike, Sachiko and Tsuruoka, Chizuru and Wada, Mami and Okayasu, Ryuichi and Ando, Koichi and Furusawa, Yoshiya and et.al and 松本 孔貴 and 鵜澤 玲子 and 平山 亮一 and 小池 幸子 and 鶴岡 千鶴 and 和田 麻美 and 岡安 隆一 and 古澤 佳也},
 month = {Sep},
 note = {Purpose: Up to 2011, over 5500 patients have been treated in HIMAC. Malignant melanoma showed high local control about 75%, whereas the overall survival was about 36% at 5-years. It is an important subject to control tumor distant metastasis for heavy-ion radiotherapy. The aim of this study is to clarify the effect of carbon ion beams (C-ions) on metastatic potential of melanoma in vitro and in vivo.  Materials and Methods: A highly metastatic mouse malignant melanoma cell line, B16/BL6 was maintained in RPMI-1640 medium supplemented with 10% FBS and antibiotics. [in vitro] Samples were prepared 1.5 days before and then irradiated with C-ions at different LETs or X-rays. Surviving fractions (SF) and metastatic potentials (migration, invasion and adhesion activity) of the cells were examined. [in vivo] Local tumors implanted into right leg of mice were also irradiated with C-ions at different LETs, or gamma-rays. The metastatic effects were analyzed with the spontaneous lung metastasis model. Radiosensitivity for individual cell in a tumor was obtained by an in vivo-in vitro assay.  Results: [in vitro] C-ions showed higher cytotoxic and anti-metastatic effects compared with X-rays, and the effects were significant depend on dose and LET values. The enhancement ratios obtained from metastatic potential were higher than that from cell killing. [in vivo] After irradiation for local tumors, the numbers of lung metastatic nodules decreased with the dose and LET values. C-ions were more effective compared with gamma-rays. The number of lung metastasis was analyzed with equivalent survival dose at D10 calculated form the survival curves of individual cell in a tumor. In this case, C-ions also showed smaller number of metastasis than gamma-rays.  Conclusion: It might suggest that C-ions inhibit metastasis at radiotherapy compared with photons., 14th International Congress of Radiation Research (ICRR2011)},
 title = {Anti-metastatic effects of carbon-ion beams for malignant tumors},
 year = {2011}
}