@misc{oai:repo.qst.go.jp:00070524,
 author = {Nakajima, Tetsuo and Bing, Wang and Nenoi, Mitsuru and 中島 徹夫 and 王 冰 and 根井 充},
 month = {Sep},
 note = {We have previously reported that low-dose-rate, long-term irradiation induces protein expression of rhodanese, a detoxification enzyme in mouse liver (Nakajima et al., J. Radiat. Res., 2008). The elucidation of the physiological role of altered protein expressions in mice irradiated at low-dose rates is important for understanding health effects from concerned radiation exposure, such as medical treatments and the high-background radiation areas. Here we tried to investigate the physiological role of the rhodanese induction by low-dose-rate irradiation using a cell line and to evaluate effects of low-dose-rate irradiation on protein expression in mouse liver using fractionated low-dose irradiation. Using rat hepatoma, McA-RH7777 cell line, which expresses rhodanese, effects of knockdown of the enzyme by siRNA on radical scavenging activities were evaluated. The siRNA treatment reduced the radical scavenging activity by 30% in the cells. This suggests that rhodanese induction by low-dose irradiation promotes radio-protective ability in mice. Furthermore, to facilitate analyses of alteration of protein expression by low-dose-rate irradiation, fractionated whole-body X-rays-irradiation was performed to mimic low-dose-rate irradiation. Mice were irradiated with 0.02Gy and 0.4Gy per day, 5 days (weekdays) per week for 1 month (total doses: 0.4Gy and 8Gy, respectively), and protein expression in livers from the mice irradiated was investigated. The male mice, 6 and 14 weeks of age (6 wk and 14 wk mice) at the start were used. In the case of 6wk mice, rhodanese induction was not observed but changes in some other protein expressions were detected in comparative analyses by two-dimensional gel electrophoresis. The proteins were identified by MALDI-TOF MS. In 14 wk mice, rhodanese induction was detected in some of mice irradiated. Moreover, as an increased protein candidate by irradiation, an identical protein was detected in both 6 wk and 14 wk mice. Characterization of protein candidates detected in altered protein expression by low-dose-rate irradiation will be discussed., 第１４回国際放射線研究会議（14th International Congress of Radiation　Research)},
 title = {Detection and characterization of proteomic alterations in the liver of mice irradiated at low-dose-rates},
 year = {2011}
}