@misc{oai:repo.qst.go.jp:00070505,
 author = {Fujimori, Akira and Hirakawa, Hirokazu and Maruyama, Futaba and Nakajima, Nakako and Yajima, Hirohiko and Kato, Takamitsu and Okayasu, Ryuichi and et.al and 藤森 亮 and 平川 博一 and 丸山 二葉 and 中島 菜花子 and 矢島 浩彦 and 加藤 宝光 and 岡安 隆一},
 month = {Sep},
 note = {Accelerated ion particle with higher LET (linear energy transfer) is promising for the treatment of certain kinds of malignancy. Molecular basis of its advantage over conventional radiotherapy, however, has not been clarified. Using HiCEP (high-coverage expression profiling), we compared gene expression profiles in the normal diploid human fibroblasts (HFLIII) irradiated with three types of clinically relevant radiation at 2Gy; X-ray (0.9 Gy/min), and carbon ion beam of either low (13 keV/micro-m) or high (70 keV/micro-m) LET. RNA was extracted at 2, 4 and 6 hr later and the expression levels for approximate 16,000 transcripts were compared between samples irradiated and mock-irradiated. Through all the nine comparison analyses, we selected 11 IR-responsive genes (signatures), which included upregulated transcripts, (BTG2, ATF3, CDKN1Aetc.) and also downregulated genes, CCNF (Cyclin F) and ASPM (abnormal spindle-like microcephaly associated). Further comparison study demonstrated that carbon particles of higher LET prolong the expression of earlier-responding genes particularly among those which are known to be upregulated under p53 control. Since it was documented that both CCNF and ASPM are required for normal mitotic-spindle formation in somatic cells, our present results would provide a novel insight into the carbon ion beams causing the significant reduction in these spindle-mitotic proteins on DNA damage response., 14 th ICRR},
 title = {Mitotic-spindle associated genes are among the common genes responding to ionizing radiation},
 year = {2011}
}