@misc{oai:repo.qst.go.jp:00070495,
 author = {Fujita, Mayumi and Yamada, Shigeru and Imai, Takashi and 藤田 真由美 and 山田 滋 and 今井 高志},
 month = {Sep},
 note = {Purpose: The aim of this study was to compare the effects of X-ray or carbon-ion (C-ion) irradiation on invasive potential of human pancreatic cancer cell line, MIAPaCa-2.
 Experimental procedures: The human pancreatic cancer cell line, MIAPaCa-2 was irradiated with either X-ray (0, 2, or 4 Gy) or C-ion (0, 0.5, 1, 2, and 4 Gy). We examined the migration, invasion, MMP expression and invasiveness in response to MMP inhibitors and/or ROCK inhibitors of irradiated cells, and the activation of Rac1 and Rho.
Summary of data: X-ray irradiation stimulated MIAPaCa-2 invasiveness via induction of MMP-2 activity, whereas C-ion irradiation reduced invasion with decreased expression and activation of MMP-2. The use of MMP-2 inhibitor (MMPI) reduced X-ray induced invasiveness suggested that M M! P-2 has role in X-ray induced invasion. However the single use of MMPI did not reduce basal level of invasiveness. ROCK inhibitor in addition to MMP-2 inhibitor was needed to suppress X-ray-induced and basal level of invasiveness indicated that both mesenchymal and amoeboid modes of motility were functioned in MIAPaCa-2 invasion. Thus, we next hypothesized that both modes of motility were inhibited in the C-ion irradiated cells, causing the effective reduction of invasiveness. In fact, the activation of Rac1 and Rho, the key factors functioned in the signal transduction pathway involved in mesenchymal or amoeboid modes of motility, were both diminished in C-ion-irradiated cells, whereas those activations were not reduced in X-ray irradiated cells.
Conclusions: X-ray and C-ion irradiation show differential effects on the invasive potential of MIAPaCa-2 cells with corresponding alterations in the MMP-2 activity and al so the activation of Rac1 and Rho signaling. Unlike X-ray irradiation, C-ion irradiation effectively suppresses the invasive potential of MIAPaCa-2 cells., 14th International Congress of Radiation Research(ICRR’2011)},
 title = {The effects of irradiation on invasive potential of human pancreatic cancer cell line, MIAPaCa-2},
 year = {2011}
}