{"created":"2023-05-15T14:51:33.243070+00:00","id":70473,"links":{},"metadata":{"_buckets":{"deposit":"804453f0-69d9-4632-8b60-e9a8922d5143"},"_deposit":{"created_by":1,"id":"70473","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70473"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070473","sets":["10:28"]},"author_link":["692075","692076","692080","692079","692074","692073","692077","692078","692081"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2011-06-08","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objectives: We have recently developed the radiobromine-labeled 5-bromo 4'-thio-2'-deoxyuridine (BTdU) as an imaigng agent for tumor proliferation. The aim of this study was to evaluate the potential usefulness of radiobromine-labeled BTdU as an imaging agent for detecting the early therapeutic effects of 5-fluorouracil (5-FU) compared with 3H-FLT uptake. \n\\nMethods: The radiolabeling of BTdU with 77Br was achieved by a destannylation reaction of tributylstannyl precursor. 3H-FLT was commerically available. The cells of human renal cacinoma cell line, ACHN, were treated with various concentrations of 5-FU (0.01-300 µM). At 36 hr after 5-FU addition, double tracer cell uptake studies were performed. 2.0x10-2 MBq of 77Br-BTdU and 3.7x10-2 MBq of 3H-FLT were simaltaneously added to the cells and incubated for 15min, 30min, 60min. After 3 times washing, cells were lysed, and then the radioactivity and protein content were measured. \n\\nResults: The radiochemical yield of 77Br-BTdU was about 40% and the radiochemical purity was more than 99%. Uptakes of 77Br-BTdU and 3H-FLT were increased with time. There was not significant differences between the uptake of 77Br-BTdU and that of 3H-FLT in control group at 60min (77Br-BTdU: 18.5 +- 2.0,3H-FLT: 20.3 +- 2.7 %ID/mg protein). Compared with control the uptake of 77Br-BTdU was significantlty decreased from 0.3 µM of 5-FU (One-way ANOVA with a post hoc Dunnett's Multiple Comparison Test). However, the uptake of 3H-FLT was significantly decreased from 300 µM of 5-FU. These results suggest that 77Br-BTdU could detect the earlier effect of 5-FU compared with 3H-FLT. \n\\nConclusions: Radiobromine-labeled BTdU is potentially useful as an imaging agent for detecting the early therapeutic effects of anticancer therapy with 5-FU in patients with renal cell carcinoma","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"SNM 2011 Annual Meeting","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takahara, Noriko"}],"nameIdentifiers":[{"nameIdentifier":"692073","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kiyono, Yasushi"}],"nameIdentifiers":[{"nameIdentifier":"692074","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshii, Yukie"}],"nameIdentifiers":[{"nameIdentifier":"692075","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujibayashi, Yasuhisa"}],"nameIdentifiers":[{"nameIdentifier":"692076","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"692077","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高原 典子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"692078","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"清野 泰","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"692079","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉井 幸恵","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"692080","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤林 康久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"692081","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Evaluation of radiobromine-labeled 5-bromo-4'-thio-2'-deoxyuridine uptake in ACHN cells after treatment with 5-fluorouracil: comparison with 3H-FLT uptake.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Evaluation of radiobromine-labeled 5-bromo-4'-thio-2'-deoxyuridine uptake in ACHN cells after treatment with 5-fluorouracil: comparison with 3H-FLT uptake."}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-07-08"},"publish_date":"2011-07-08","publish_status":"0","recid":"70473","relation_version_is_last":true,"title":["Evaluation of radiobromine-labeled 5-bromo-4'-thio-2'-deoxyuridine uptake in ACHN cells after treatment with 5-fluorouracil: comparison with 3H-FLT uptake."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:02:33.123206+00:00"}