@misc{oai:repo.qst.go.jp:00070240,
 author = {Hasegawa, Sumitaka and Saito, Shigeyoshi and Morokoshi, Yukie and Furukawa, Takako and Aoki, Ichio and Saga, Tsuneo and 長谷川 純崇 and 齋藤 茂芳 and 諸越 幸恵 and 古川 高子 and 青木 伊知男 and 佐賀 恒夫},
 month = {Sep},
 note = {Purpose: To develop a selective diagnostic imaging of mesothelioma by targeting disease-specific biomarkers.
Materials and Methods: All experiments were approved by the Institutional Animal Care and Use Committee. Manganese-superoxide dismutase (Mn-SOD) expression was evaluated in one human mesothelial cells and five human malignant mesothelioma (MM) cells. Mn accumulation in NCI-H226 and MSTO-211H MM cells was examined when loaded with Mn. Mn-enhanced magnetic resonance imaging (MEMRI) of those cell pellets and subcutaneous tumors were conducted using 7 Tesla-MRI. Signal enhancement of H226 xenografted pleural tumors was determined by MEMRI with manganese dipyridoxyl diphosphate (MnDPDP) as well as manganese chloride (MnCl2). 
Results: We found that 4 of 5 human MM cells over-expressed Mn-SOD protein compared to mesothelial cells, and that H226 MM cells highly expressed Mn-SOD and augmented Mn accumulation when loaded with MnCl2. The cells showed marked T1-signal enhancement on in vitro MRI after incubation with MnCl2. H226 subcutaneous tumors were preferentially enhanced compared to MSTO-211H tumors, which had less Mn-SOD expression, in MnCl2-enhanced T1-weighted MR image (T1WI). H226 pleural tumors were markedly enhanced and readily detected by MEMRI using MnDPDP as well as MnCl2.
Conclusion: We propose that MEMRI can be a potentially powerful method for non-invasive detection of MM with high spatial resolution and marked signal enhancement by targeting Mn-SOD., World  Molecular Imaging Congress 2010},
 title = {Manganese-enhanced MRI as a molecular imaging of mesothelioma},
 year = {2010}
}