{"created":"2023-05-15T14:51:22.254689+00:00","id":70227,"links":{},"metadata":{"_buckets":{"deposit":"00285946-73f1-4434-85e4-4878cf65d97f"},"_deposit":{"created_by":1,"id":"70227","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70227"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070227","sets":["10:28"]},"author_link":["689551","689552","689545","689549","689542","689541","689544","689540","689548","689550","689543","689546","689553","689547"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-09-11","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The catecholamine, dopamine (DA), is synthesized from 3,4-dihydroxy-L-phenylalanine (L-DOPA) by aromatic L-amino acid decarboxylase (AADC). DA metabolism is regulated by monoamine oxidase (MAO) and catechol-O-methyltransferase (COMT). To measure dopaminergic metabolism, we used microdialysis with radiometric detection to monitor L-[beta-11C]DOPA metabolites in the extracellular space of the rat striatum and plasma. We also evaluated the effects of AADC, MAO, and COMT inhibitors on striatal metabolite profiles. \nL-[beta-11C]DOPA and its radioactive metabolites, [11C]3,4-dihydroxyphenylacetic acid ([11C]DOPAC), [11C]homovanillic acid ([11C]HVA), L-3-O-methyl-[11C]DOPA ([11C]3-OMD) and [11C]3-methoxytyramine ([11C]3-MT) were detected, however [11C]DA was not detected in striatal dialysate following intravenous injection of L-[beta-11C]DOPA. The major early species measured after administration of L-[beta-11C]DOPA were [11C]DOPAC and [11C]HVA in a 1:1 ratio, which shifted toward [11C]HVA with time. An AADC inhibitor increased the uptake of L-[beta-11C]DOPA and [11C]3-OMD and delayed the accumulation of [11C]DOPAC and [11C]HVA. With MAO inhibition, about 80% of total radioactivity was derived from [11C]3-MT. The COMT inhibitor increased [11C]DOPAC and decreased [11C]HVA. L-[beta-11C]DOPA and [11C]3-OMD were detected and then achieved equilibrium in plasma with AADC inhibitor. On the other hand, [11C]3-OMD levels in striatal dialysate decreased significantly following intrastriatal administration of L-[beta-11C]DOPA. These results suggest that [11C]3-OMD in striatal dialysate was generated mostly in peripheral tissue and penetrate into brain. \nOur results reflect the complicated L-DOPA metabolic pathway, suggesting that this method could improve the understanding of the information obtained from an L-[beta-11C]DOPA PET study or provide an index of dopaminergic metabolism-related neuropsychiatric disorders and evaluation of drug therapies for treatment of the disorder.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"2010 World Molecular Imaging Congress","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Okada, Maki"}],"nameIdentifiers":[{"nameIdentifier":"689540","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakao, Ryuji"}],"nameIdentifiers":[{"nameIdentifier":"689541","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hosoi, Rie"}],"nameIdentifiers":[{"nameIdentifier":"689542","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Zhang, Ming-Rong"}],"nameIdentifiers":[{"nameIdentifier":"689543","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fukumura, Toshimitsu"}],"nameIdentifiers":[{"nameIdentifier":"689544","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Kazutoshi"}],"nameIdentifiers":[{"nameIdentifier":"689545","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Inoue, Osamu"}],"nameIdentifiers":[{"nameIdentifier":"689546","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡田 真希","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689547","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中尾 隆士","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689548","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"細井 理恵","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689549","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"張 明栄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689550","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"福村 利光","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689551","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木 和年","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689552","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"井上 修","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"689553","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Microdialysis with radiometric monitoring of L-[beta-11C]DOPA metabolites in rat striatum and plasma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Microdialysis with radiometric monitoring of L-[beta-11C]DOPA metabolites in rat striatum and plasma"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-09-13"},"publish_date":"2010-09-13","publish_status":"0","recid":"70227","relation_version_is_last":true,"title":["Microdialysis with radiometric monitoring of L-[beta-11C]DOPA metabolites in rat striatum and plasma"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:05:24.597858+00:00"}