@misc{oai:repo.qst.go.jp:00070214,
 author = {Isono, Mayu and Shinya, Fuyumi and Otsu, Masahiro and Konishi, Teruaki and Nakayama, Takashi and Inoue, Nobuo and 小西 輝昭},
 month = {Sep},
 note = {The neural stem cells (NSCs) increase in the number and differentiate into neurons, astrocytes and oligodendrocytes in developmental brain during the fetal period. Radiation exposure in the period leads to disturbances in development of the brain and can result in microcephalia and other neurological dysfunctions such as mental retardation. We have demonstrated that X-irradiation of the NSCs, prepared from mouse embryonic stem (ES) cells by the NeuralStem Sphere method, inhibits proliferation dose-dependently. In this study, we investigated effects of X-irradiation of NSCs on proliferation and differentiation into the neural cells. When proliferating NSCs were irradiated with 1 Gy, the cells stopped proliferation but started to proliferate exponentially with a lag time of about 1 day. The cells expressed nestin and maintained potential of proliferation and differentiation like non-irradiated cells. When the NSCs were irradiated with 5 Gy, the cells stopped proliferation and became larger in size. The cells expressed nestin, however, lost potential of proliferation and differentiation. These results suggest that radiation-induced damage can be repaired in the NSCs irradiated with 1 Gy but not in the cells with 5 Gy and over., Neuro2010},
 title = {Effects of X-irradiation on proliferation and differentiation of neural stem cells derived from mouse embryonic stem cells},
 year = {2010}
}