{"created":"2023-05-15T14:51:19.408903+00:00","id":70162,"links":{},"metadata":{"_buckets":{"deposit":"98964b49-069b-4b9c-9023-193571a31f84"},"_deposit":{"created_by":1,"id":"70162","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70162"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070162","sets":["10:28"]},"author_link":["688946","688947","688940","688945","688948","688950","688951","688949","688952","688944","688941","688943","688942","688939"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-05-22","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: Up to 2009, over 5000 patients have been treated in HIMAC. Malignant melanoma showed high local control of about 75%, whereas the overall survival was about 36% at 5-years. It is an important subject to control tumor distant metastasis for heavy-ion radiotherapy. The aim of this study is to clarify the effect of carbon ion beams (C-ion) on metastatic potential of melanoma in vitro and in vivo. \nMaterials and Methods:  A highly metastatic mouse malignant melanoma cell line B16/BL6 were maintained in RPMI-1640 medium supplemented with 10% FBS and antibiotics. [in vitro] Samples were prepared 2 days before and then irradiated with C-ions at 13, 50 or 75 keV/um or X-rays. Surviving fractions were obtained using colony formation assay. Migration and invasion activity as metastatic potentials of the cells were examined using the Boyden-chamber method and the Matrigel invasion assay. [in vivo] The cells were implanted in right-leg of C57BL/6J mice at 1x106 cells/mouse 9-10 days before irradiation. Tumors were irradiated at the center of 6cm-SOBP of C-ions, or gamma-rays. Radiosensitivity for whole tumor was obtained by the tumor growth delay method, and that for individual cell in a tumor was obtained by an in vivo-in vitro assay. The metastatic effects were analyzed with the spontaneous lung metastasis model. \nResults: [in vitro] Survival curves showed higher cytotoxic effects of C-ions compared with X-rays, and the RBE values were 1.1, 1.7 or 2.5 at 13, 50, or 75 keV/um, respectively. The potential of migration and invasion were suppressed by C-ions at all dose points (0.25 to 8.0 Gy) tested, however it was enhanced by X-rays at low dose points (0.25 to 0.5Gy) than non-irradiated controls. The RBE values obtained from migration and invasion test were higher than that from cell killing.  [in vivo] C-ions significantly suppressed the tumor growth, and the RBE was 2.6.  The numbers of lung metastatic nodules after tumor-irradiations decreased with the dose, and C-ions were more effective compared with gamma-rays.  The metastatic potentials of survived cells in a tumor after irradiation was analyzed with the number of metastatic lung colony from implanted tumors and survival of irradiated and explanted cells from a tumor.  \nSmaller number of metastasis was found for C-ions than gamma-rays when the tumor cell survivals were 10%.  \nConclusion:  It might suggest that C-ion inhibit metastasis at radiotherapy compared with low-LET photons.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"PTCOG 49","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Matsumoto, Yoshitaka"}],"nameIdentifiers":[{"nameIdentifier":"688939","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koike, Sachiko"}],"nameIdentifiers":[{"nameIdentifier":"688940","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Uzawa, Akiko"}],"nameIdentifiers":[{"nameIdentifier":"688941","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirayama, Ryoichi"}],"nameIdentifiers":[{"nameIdentifier":"688942","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"688943","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ando, Koichi"}],"nameIdentifiers":[{"nameIdentifier":"688944","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furusawa, Yoshiya"}],"nameIdentifiers":[{"nameIdentifier":"688945","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"688946","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本 孔貴","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"688947","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小池 幸子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"688948","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鵜澤 玲子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"688949","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平山 亮一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"688950","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"688951","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古澤 佳也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"688952","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"The effects of heavy-ion and photon beams to mouse malignant melanoma cell line having highly metastatic potential","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The effects of heavy-ion and photon beams to mouse malignant melanoma cell line having highly metastatic potential"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-06-22"},"publish_date":"2010-06-22","publish_status":"0","recid":"70162","relation_version_is_last":true,"title":["The effects of heavy-ion and photon beams to mouse malignant melanoma cell line having highly metastatic potential"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:06:08.606972+00:00"}