{"created":"2023-05-15T14:51:16.368382+00:00","id":70092,"links":{},"metadata":{"_buckets":{"deposit":"be4b6b56-455d-4b00-a3b1-7aabe66d4add"},"_deposit":{"created_by":1,"id":"70092","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"70092"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00070092","sets":["10:28"]},"author_link":["688317","688312","688313","688314","688316","688315"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-05-06","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A paramagnetic nitroxyl radical was oxidized by superoxide (•O2-) or hydroxyl radical (•OH) to give the oxoamonium cation. The oxoamonium cation reacts with glutathione to make a stable diamagnetic compound (structure unidentified) or with NAD(P)H to become the hydroxylamine. The hydroxylamine can be re-oxidized mainly by •OH to give the corresponding nitroxyl radical. Hydrogen peroxide (H2O2) participates a reducing the nitroxyl radical during high dose irradiation, since this reduction of nitroxyl radical was restrained by catalase. In this study, amount and distribution of several ROS generated in a gelatin sample by carbon ion irradiation was estimated using a nitroxyl radical, 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPOL), or its hydroxylamine form, i.e. TEMPOL-H as a redox probe.\nThe reduction of TEMPOL in a reaction mixture containing a combination of TEMPOL and GSH can be an index of total reaction of ROS (mainly •OH and •O2-). The GSH-independent reduction of nitroxyl radical caused by high dose irradiation in a reaction mixture of TEMPOL alone can be an index of H2O2 generation. The oxidation of TEMPOL-H in a reaction mixture containing TEMPOL-H alone can be an index of OH. Each reaction mixture containing 0.1 mM TEMPOL or TEMPOL-H was caked using gelatin to test depth-dependent redox reactions. The gelatin sample was irradiated with a 290 MeV/nucleon carbon ion beam at a dose of 32 Gy with several different linear energy transfer (LET) setting. An aliquot of gelatin sample was sampled from surface using a glass capillary, and then increase or decrease of TEMPOL signal was measured by X-band EPR spectrometer.\nAmount of GSH-dependent reduction of TEMPOL was 400–1200 nmol/L/Gy, which decreased with increasing LET. Amount of GSH-independent reduction of TEMPOL was 30–300 nmol/L/Gy, which also decreased with increasing LET. The level of 30–300 nmol/L/Gy GSH-independent reduction of TEMPOL was estimated as around 60–600 nmol/L/Gy of H2O2 generation if it was simply correlated with X-ray. Amount of oxidation of TEMPOL-H was 2–4 nmol/L/Gy, which also decreased with increasing LET. This result is suggesting that •O2- and/or H2O2 may be an important species to regulate at lower LET part, which usually corresponds to a normal tissue at the carbon ion cancer therapy.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"EPR 2010 (A Joint Conference of the 14th In Vivo EPR Spectroscopy and Imaging and the 11th International EPR Spin Trapping/Spin Labeling)","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Matsumoto, Kenichiro"}],"nameIdentifiers":[{"nameIdentifier":"688312","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakanishi, Ikuo"}],"nameIdentifiers":[{"nameIdentifier":"688313","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Anzai, Kazunori"}],"nameIdentifiers":[{"nameIdentifier":"688314","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本 謙一郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"688315","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中西 郁夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"688316","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安西 和紀","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"688317","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Reactive Oxygen Species Generated in an Aqueous Gelatin Sample Irradiated by Carbon Ion Beam","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Reactive Oxygen Species Generated in an Aqueous Gelatin Sample Irradiated by Carbon Ion Beam"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-05-10"},"publish_date":"2010-05-10","publish_status":"0","recid":"70092","relation_version_is_last":true,"title":["Reactive Oxygen Species Generated in an Aqueous Gelatin Sample Irradiated by Carbon Ion Beam"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:06:55.185660+00:00"}