{"created":"2023-05-15T14:51:12.283369+00:00","id":69998,"links":{},"metadata":{"_buckets":{"deposit":"5a5ddf22-788f-47dc-a0bf-63dcf032a08a"},"_deposit":{"created_by":1,"id":"69998","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"69998"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00069998","sets":["10:28"]},"author_link":["687268","687266","687270","687273","687274","687267","687271","687275","687272","687269"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2009-12-12","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Villin1 (VIL1), a major structural component of the brush border cytoskeleton, binds actin in a calcium-dependent manner and has been reported as a marker for colon cancers. VIL1 was recently found to be an epithelial cell-specific anti-apoptotic protein and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transition, cell migration and cell survival. \n\\nPurpose:  We investigated a novel molecular biomarker for cervical adenocarcinoma (AD) through the integration of multiple methods of genomic analysis. \nMaterials and Methods: Tumor samples in discovery set were obtained from 87 patients who underwent radiotherapy, including 31 cervical AD. Microarray analysis and quantitative polymerase chain reaction analysis were performed to screen a candidate diagnostic molecule for cervical AD, and its clinical significance was investigated by immunohistochemical analysis (IHC). \nResults: We analyzed the expression profiles of genes mapping to common deletious region in cervical cancer, and identified several consistently down-regulated genes, including ERBB4, VIL1, and DES in squamous cell carcinoma (SCC). Furthermore, we found a difference between biopsy samples of AD and SCC in the expression and genomic copy number of VIL1, which maps to 2q35. IHC revealed 14 VIL1-positive tumors; 13 cervical AD and 1 small cell carcinoma of cervix, while none of SCC or endometrial AD was VIL1-positive. The marker was validated by newly enrolled 65 patients, and VIL1 positive staining showed 52% of sensitivity and 100% of selectivity for cervical AD. In conclusion, we have identified VIL1 as a novel biomarker of cervical AD.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"The 32nd Annual Meeting of the Molecular Biology Society of Japan","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakamura, Etsuko"}],"nameIdentifiers":[{"nameIdentifier":"687266","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Iwakawa, Mayumi"}],"nameIdentifiers":[{"nameIdentifier":"687267","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakawatari, Miyako"}],"nameIdentifiers":[{"nameIdentifier":"687268","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imadome, Kaori"}],"nameIdentifiers":[{"nameIdentifier":"687269","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imai, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"687270","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中村 悦子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"687271","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岩川 眞由美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"687272","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中渡 美也子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"687273","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今留 香織","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"687274","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井 高志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"687275","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Actin-binding protein, VIL1, in cervical adenocarcinomas","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Actin-binding protein, VIL1, in cervical adenocarcinomas"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-12-15"},"publish_date":"2009-12-15","publish_status":"0","recid":"69998","relation_version_is_last":true,"title":["Actin-binding protein, VIL1, in cervical adenocarcinomas"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:08:00.065169+00:00"}