@misc{oai:repo.qst.go.jp:00069986,
 author = {Fujita, Kazuko and Bing, Wang and Kuwabara, Taku and Tanaka, Kaoru and Kamata, Itaru and Akasaka, Yoshikiyo and Ishii, Hisaharu and 藤田 和子 and 王 冰 and 桑原 卓 and 田中 薫},
 month = {Dec},
 note = {CD180 molecule, a homologue of TLR-4, expresses on mature B cells.
A large number of B cells in peripheral blood express CD180 on surface. It
was reported that CD180-negative B cells increase in peripheral blood from
patients with autoimmune diseases such as SLE, a number of CD180-negative
cells is reflected a stage of the disease, and CD180-negative cells from SLE
patients produce autoantibodies.  These results suggest that the CD180
expression on B cells was strongly concerned with autoimmune reaction. We
previously showed that CD180-negative spleen B cells were more
radiosensitive than CD180-positive cells with regard to apoptosis. In this
study, we attempted to demonstrate the relationship between CD180 expression
and autoimmune reaction in an animal SLE model, the NZBWF1 mice. Animals
attained the criteria of disease occurrence were paired according to body
weight and other conditions.  One of the pair was irradiated with 4 Gy of
X-rays, and the other sham-irradiated.  After irradiation, the life span of
the irradiated mice was significantly increased than that of the
sham-irradiated mice and same as paired mice. The amount of complement C3 in
peripheral blood, which decreases as the disease, of the irradiated mice was
markedly higher than their sham-irradiated mice at 3 weeks after
irradiation. By immunohistological examination of kidneys, number of
CD180-negative cells of SLE mice increased compared with that before
disease.  The findings support the conception that radiation could
effectively eliminate CD180-negative B cells, leading to the reduced
production of autoantibodies, and thus relieve SLE symptoms. The findings
suggest the possibility of practical application of radiotherapy to
autoimmune diseases.
CD180 molecule, a homologue of TLR-4, expresses on mature B cells.
A large number of B cells in peripheral blood express CD180 on surface. It
was reported that CD180-negative B cells increase in peripheral blood from
patients with autoimmune diseases such as SLE, a number of CD180-negative
cells is reflected a stage of the disease, and CD180-negative cells from SLE
patients produce autoantibodies.  These results suggest that the CD180
expression on B cells was strongly concerned with autoimmune reaction. We
previously showed that CD180-negative spleen B cells were more
radiosensitive than CD180-positive cells with regard to apoptosis. In this
study, we attempted to demonstrate the relationship between CD180 expression
and autoimmune reaction in an animal SLE model, the NZBWF1 mice. Animals
attained the criteria of disease occurrence were paired according to body
weight and other conditions.  One of the pair was irradiated with 4 Gy of
X-rays, and the other sham-irradiated.  After irradiation, the life span of
the irradiated mice was significantly increased than that of the
sham-irradiated mice and same as paired mice. The amount of complement C3 in
peripheral blood, which decreases as the disease, of the irradiated mice was
markedly higher than their sham-irradiated mice at 3 weeks after
irradiation. By immunohistological examination of kidneys, number of
CD180-negative cells of SLE mice increased compared with that before
disease.  The findings support the conception that radiation could
effectively eliminate CD180-negative B cells, leading to the reduced
production of autoantibodies, and thus relieve SLE symptoms. The findings
suggest the possibility of practical application of radiotherapy to
autoimmune diseases., 第39回日本免疫学会総会・学術集会、},
 title = {Induced Life Extension in SLE-Model Mice by Irradiation that Selectively Eliminated CD180-Negative B Cells.},
 year = {2009}
}