@misc{oai:repo.qst.go.jp:00069919,
 author = {Murai, Chika and Inubushi, Masayuki and Hata, Hironobu and Shidahara, Miho and Sogawa, Chizuru and Tsuji, Atsushi and Koizumi, Mitsuru and Kitagawa, Yoshimasa and Saga, Tsuneo and 村井 知佳 and 犬伏 正幸 and 志田原 美保 and 曽川 千鶴 and 辻 厚至 and 小泉 満 and 佐賀 恒夫},
 month = {Oct},
 note = {Aim: The purposes of this study were to establish cancer cell lines stably expressing human sodium-iodide symporter (hNIS), and to investigate characteristics of their relevant xenograft models in nude mice by SPECT/CT imaging.
Material and Method: A recombinant plasmid containing hNIS (pcDNA3-hNIS) was transfected into a human colon cancer cell line HCT116 by the calcium phosphate coprecipitation method, and 5 stably transformed cell lines (A, B, C, D, E) were isolated by G418 selection. Their functional hNIS expression was confirmed by 99mTcO4- uptake tests in vitro. Three lines (A, B, E) of them and negative control HCT116 (N) were subcutaneously injected into four regions of 16 nude mice. The rate and time of tumor formation (>=8 mm) were observed, and the tumor diameter was measured every 2 to 3 days. When the tumor diameter was between 8 and 13 mm, 99mTcO4- SPECT imaging was performed using a dedicated small animal SPECT system FX(SPECT/CT) (Gamma Medica-Ideas, CA) to estimate tumor 99mTcO4- uptake in percent injection dose per gram (%ID/g).
Result: The 16 mice formed 11 tumors of cell line A (69%), 10 of line B (63%), 14 of line E (94%), and 14 of control N (88%) (p=NS). The time of tumor formation was 36+/-9(mean+/-SD) days in line A, 24+/-8 days in line B, 26+/-12 days in line E, and 18+/-12 days in control N (p<0.05 for A vs N; otherwise p=NS). The SPECT image quality was good, and all the tumors of cell lines A, B and E were well visualized. The average of tumor 99mTcO4- uptake in %ID/g was 25.7+/-8.3 to line A tumors, 35.9+/-12.3 to line B tumors, 11.6+/-6.4 to line E tumors, and 2.4+/-0.9 to control N tumors (p<0.001 for A vs N and for B vs N; p<0.01 for B vs E; otherwise p=NS). These results indicate the cell line B has the best characteristics of preserved rapid growth rate and the highest 99mTcO4- uptake for SPECT imaging.
Conclusion: We established the cancer cell line stably expressing hNIS (HCT116-hNIS line B), and succeeded in imaging the mouse xenograft model by 99mTcO4- SPECT. In the future, this model will provide a tool to track tumor metastasis in vivo in mice so that the molecular mechanism involved in metastatic cascade will be elucidated., Annual Congress of the European Association of Nuclear Medicine 2009},
 title = {Radionuclide reporter gene imaging of mouse xenograft model of human colon cancer cell lines stably expressing human sodium-iodide symporter (hNIS)},
 year = {2009}
}