@misc{oai:repo.qst.go.jp:00069918,
 author = {Iwakawa, Mayumi and Nakawatari, Miyako and Imadome, Kaori and Ohno, Tatsuya and Kato, Shingo and Nakamura, Etsuko and Sakai, Minako and Ohkubo, Yu and Tamaki, Tomoaki and Imai, Takashi and 岩川 眞由美 and 中渡 美也子 and 今留 香織 and 大野 達也 and 加藤 眞吾 and 中村 悦子 and 酒井 美奈子 and 大久保 悠 and 田巻 倫明 and 今井 高志},
 month = {Oct},
 note = {Purpose
Tumor microenvironment possesses extreamly important role for tumor progression and metastasis. Cytokines have autocrine and paracrine functions, and they are also secreted by normal and cancerous cells. Herewith we investigated an indicator for the efficacy of radiotherapy in cervical cancers (CC) using microarray analysis and immunohistochemical analysis.
Patients and methods
One hundred and four patients with CC were recruited and divided into two groups (research set: n =35, and validation set: n =69). Microarray analysis was performed in research set and further immunohistochemical analysis (IHA) was performed for all patients to detect candidate radioresponsive markers using pre-radiotherapy and mid-radiotherapy biopsy samples, which were taken one week after initiation of radiotherapy.
Results
 FGF2 in tumor cells (FGF2–T) significantly increased in midtreatment samples compared with pretreatment samples in research set of patients, and the ratio change of FGF2-T was significantly related with better prognosis. This evidence was confirmed in validation set. Next using all 104 patimets we found IHA positive FGF2 in stromal cells (FGF2-S) in 85 patients, and the radiotherapy-induced increase of FGF-S in 23 patients. Though positive FGF2-S in pretreatment samples was significantly related with increased expression change of VEGF, it was not related with poor prognosis.
Conclusion
Radiation causes severing the normal or cancerous associations with adjacent cells and changes the extracellular matrix environment. Therefore, we need to investigate not only pretreatment status of tumors, but also modified tumor structures during fractionated radiotherapy. In this study, we found FGF2-T expression change as a monitoring marker for the effectiveness of radiotherapy, and found the relationship between FGF2-S in pretreatment status and VEGF expression change in a subgroup of patients., 5th International Conference on Tumor Microenvironment: Progression, Therapy & Prevention},
 title = {FGF2 expression change as an acute radiotherapy responsive marker in sequential biopsy samples from cervical cancer patients during fractionated radiotherapy.},
 year = {2009}
}