@misc{oai:repo.qst.go.jp:00069874,
 author = {Kimura, Yuichi and Seki, Chie and Nobuya, Hashizume and Nishimoto, Takahiro and Kitamura, Keishi and Kanno, Iwao and 木村 裕一 and 関 千江 and 橋爪 宣弥 and 北村 圭司 and 菅野 巖},
 month = {Sep},
 note = {[Introduction] The system for uL-ordered blood sampling and plasma radioactive
concentration measurement, Microfluidic Mouse Plasma Counting System (uFmPC, patent pending), is
developing. uFmPC should measure very small radioactivity concentration, from several to hundreds Bq/uL.
This study aimed to investigate the accuracy on such small radioactivity concentration. [Method] In uFmPC,
blood was sampled using microfluidic technique, and it was stocked in channels on a plastic disc (CD-Well).
The blood was then separated by rotating the disc. Because the channels had exact cross-sectional area of
0.067 mm2, the volumes of blood and separated plasma could be derived by measurement of the length
using a flatbed scanner. The amount of radioactivity was acquired by a storage phosphor screen. In the
experiments, one and three uL of FDG solutions containing the radioactivity ranging from 4 to 128 Bq/uL
were introduced in the channels. The radioactivity was measured using Imaging Plate (IP) and BAS-5000
(Fujifilm Corp., Japan) with 120-minute exposure. CD-Well was also scanned by GT-X970 scanner (Seiko
EPSON, Japan) in 2400 dpi in order to measure the volume of solution. The activity concentration of given
FDG was compared with a photo-stimulated luminescence value (PSL) derived from uFmPC. [Results and
Discussion] Measured images were shown in Fig. 1: a scanned image (A) and an image from IP (B). The
position of introduced FDG was detected as shown in (C) with black thick curves, and it was applied to the
IP image to read out an amount of radioactivity. (D) was a superimposed image. From 120-minute exposure,
the PSL was correlated with the given concentration well (y = 1.8x + 4.4 for 1 uL case and y = 2.1x + 6.6 for
3 uL case. r2=1.00 for both cases). We can conclude that the concept of uFmPC had potential to measure
small radioactivity concentration., 2009 World Molecular Imaging Congress},
 title = {Development of Microfluidic Plasma Counting System for Small Animal Molecular Imaging Using PET --- Measurement of Small Radioactivity Concentration},
 year = {2009}
}