{"created":"2023-05-15T14:51:00.510104+00:00","id":69728,"links":{},"metadata":{"_buckets":{"deposit":"508f2383-2c2a-4e5b-bf0f-7a9b2b92c2fe"},"_deposit":{"created_by":1,"id":"69728","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"69728"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00069728","sets":["10:28"]},"author_link":["684521","684524","684520","684528","684525","684523","684526","684522","684519","684527","684530","684529"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2009-03-17","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"現在市販されている緑色蛍光（GFP）マウスは、生物医学研究に多用されている。しかしながら、GFP単色のみでは、臓器幹細胞などを、多面的に解析することは不可能である。そこで我々は、国立がんセンター研究所との共同研究により、緑色と区別しやすい赤色蛍光タンパク質（RFP）を導入したトランスジェニックマウス（DsRed-Tgマウス）を作製したので報告する。pCAGGS-DsRed2 DNAをマウス前核に注入し、14匹のDsRed+/-マウスが得られた。その中で、皮膚と白血球にて発現の強い３系統（No. 1, 5, 34）を維持、胚凍結し、発現の弱い１系統（No. 40）について精子凍結をすすめている。このDsRed-Tgマウスは、遺伝的バックグラウンドにGFPマウスと同じC67BL/6CrSlcを用いており、臓器移植実験などにてGFPマウスと併用することも可能である。本系統は、所内研究に対して提供可能なので、今後は本Tgマウスを用いた共同研究依頼に対応する所存である。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第４回技術と安全の報告会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鬼頭, 靖司"}],"nameIdentifiers":[{"nameIdentifier":"684519","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"太田, 有紀"}],"nameIdentifiers":[{"nameIdentifier":"684520","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森, 雅彦"}],"nameIdentifiers":[{"nameIdentifier":"684521","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"五十嵐, 美徳"}],"nameIdentifiers":[{"nameIdentifier":"684522","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塚本, 智史"}],"nameIdentifiers":[{"nameIdentifier":"684523","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"酒井, 一夫"}],"nameIdentifiers":[{"nameIdentifier":"684524","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鬼頭 靖司","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"684525","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"太田 有紀","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"684526","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森 雅彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"684527","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"五十嵐 美徳","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"684528","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塚本 智史","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"684529","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"酒井 一夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"684530","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"赤色蛍光遺伝子（DsRed2）トランスジェニックマウスの新規作製・系統化","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"赤色蛍光遺伝子（DsRed2）トランスジェニックマウスの新規作製・系統化"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-04-01"},"publish_date":"2009-04-01","publish_status":"0","recid":"69728","relation_version_is_last":true,"title":["赤色蛍光遺伝子（DsRed2）トランスジェニックマウスの新規作製・系統化"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:11:06.557202+00:00"}