{"created":"2023-05-15T14:50:57.865866+00:00","id":69667,"links":{},"metadata":{"_buckets":{"deposit":"0d1bcdea-9fe1-479e-b8cd-1175e85d81f1"},"_deposit":{"created_by":1,"id":"69667","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"69667"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00069667","sets":["10:28"]},"author_link":["683879","683880","683877","683878"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2008-12-12","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ヒトp280蛋白質は進化的に保存されたZnフィンガードメインとSWI/SNFタイプのヘリカーゼドメインを持っており、クロマチン構造を介して遺伝子発現を制御すると考えられるが、その生化学的な機能は不明である。我々は今までに（１）p280のZnフィンガードメインがRINGフィンガーやPHDフィンガーと弱い相同性を持つことに着目し、ユビキチンリガーゼ活性を持つこと示していた。更に、（２）p280様のZnフィンガードメインを持つ蛋白質をコードするヒトの遺伝子を新たに3つ同定し、（３）これら3つのZnフィンガードメインとmaltose-binding protein (MBP)の融合蛋白質はUbcH5に依存して自己ユビキチン化されることを示した。以上の結果よりp280様のZnフィンガードメインはいずれもユビキチンリガーゼ活性を持つことが分かった。今回、ユビキチンリガーゼ活性に重要なアミノ酸残基を同定するためにZnフィンガードメインの変異体解析を行った。その結果、p280様Znフィンガードメインで保存されたアミノ酸残基はユビキチンリガーゼ活性に重要であることがわかったので報告する。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第31回日本分子生物学会年会　第81回日本生化学会　合同大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"南雲, 利之"}],"nameIdentifiers":[{"nameIdentifier":"683877","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"瀧, 景子"}],"nameIdentifiers":[{"nameIdentifier":"683878","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"その他"}],"nameIdentifiers":[{"nameIdentifier":"683879","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"瀧 景子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"683880","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Mutational studies of a p280-like Zn finger domain revealed functional importance of conserved amino acid residues for its ubiquitin ligase activity.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Mutational studies of a p280-like Zn finger domain revealed functional importance of conserved amino acid residues for its ubiquitin ligase activity."}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-12-25"},"publish_date":"2008-12-25","publish_status":"0","recid":"69667","relation_version_is_last":true,"title":["Mutational studies of a p280-like Zn finger domain revealed functional importance of conserved amino acid residues for its ubiquitin ligase activity."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:11:48.295954+00:00"}