{"created":"2023-05-15T14:50:57.780016+00:00","id":69665,"links":{},"metadata":{"_buckets":{"deposit":"3d1679e9-d791-4cdc-aab3-91ab3ee4321d"},"_deposit":{"created_by":1,"id":"69665","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"69665"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00069665","sets":["10:28"]},"author_link":["683849","683851","683850"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2008-12-12","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"High dose radiation induces apoptosis of intestinal epithelial cells and subsequent depletion of the cells, resulting in lethal intestinal injury. However, effective treatment of this injury has not been established yet. Lithium chloride (LiCl) is well known as an inhibitor of glycogen synthase kinase 3 (GSK3), which has been shown to be associated with apoptosis. We studied the effect of LiCl on intestinal radiation injury. Rat small intestinal epithelial cell line, IEC-6 cells and intestinal epithelial cells in primary culture obtained from fetal rat duodenum were treated with LiCl for an hour and then exposed to gamma-radiation of 20 Gy. Twenty-four hours after irradiation, the apoptosis was evaluated by Hoechst staining. Pretreatment with 10 mM of LiCl markedly inhibited radiation-induced apoptosis in both cells.  Furthermore, addition of LiCl after irradiation blocked the apoptosis. Inhibition of either PI3K/Akt or MEK/ERK pathway abrogated the anti-apoptotic effect of LiCl. We administered LiCl to Balb/c mice 1 h before total-body irradiation (TBI) with 8 Gy. Administration of LiCl blocked the radiation-induced apoptosis in intestinal crypts. Moreover, either pre- or post-administration of LiCl increased the number of surviving crypts in mice 3.5-day after TBI. In the present study, we found that LiCl protects and rescues intestinal epithelial cells from radiation-induced apoptosis through activation of pathways involving PI3K/Akt and MEK/ERK.  Our results also show that LiCl prevents radiation-induced intestinal injury in vivo.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第31回日本分子生物学会年会第81回日本生化学会大会合同大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"坂口, 奈賀子"}],"nameIdentifiers":[{"nameIdentifier":"683849","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"その他"}],"nameIdentifiers":[{"nameIdentifier":"683850","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"坂口 奈賀子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"683851","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"放射線誘発小腸障害に対するリチウムの影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"放射線誘発小腸障害に対するリチウムの影響"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-12-25"},"publish_date":"2008-12-25","publish_status":"0","recid":"69665","relation_version_is_last":true,"title":["放射線誘発小腸障害に対するリチウムの影響"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:11:49.688039+00:00"}