{"created":"2023-05-15T14:50:51.700296+00:00","id":69526,"links":{},"metadata":{"_buckets":{"deposit":"f07415b1-539f-4423-9d11-a4ba30a7ed29"},"_deposit":{"created_by":1,"id":"69526","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"69526"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00069526","sets":["10:28"]},"author_link":["682311","682309","682321","682310","682322","682315","682319","682314","682316","682318","682327","682317","682323","682313","682324","682328","682325","682320","682326","682312"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2008-10-15","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aim: Gastrointestinal stromal tumor (GIST) is a most common mesenchymal tumor arising from the human gastrointestinal tract. Overexpression of c-kit, encoding type III receptor tyrosine kinase, and its functional mutation is present in most GIST. 18F-fluoro-deoxyglucose (FDG) has been reported to highly accumulate in GISTs, and positron emission tomography using FDG (FDG-PET) is very useful for tumor detection and also for the assessment of early response to treatments using Imatinib (Gleevec). However, FDG-PET, in some cases, fails to detect relapse of GIST at early time point. If we could develop a specific method to detect c-kit expression, it can be used for the evaluation of the early response and relapse of GISTs. In this study, we labeled anti-c-kit antibodies with 125I and 111In, and assessed its in vitro and in vivo characteristics.\nMaterials & methods: We constructed an expressing vector of mutated c-kit and transfected it to HEK293 human embryonic kidney cells and isolated several stable clones. To select highly expressing cell clones, we determined c-kit expression using western blot analysis and immunofluorescent staining. The cell suspension (1x107) was mixed with Matrigel, and injected subcutaneously in BALB/c-nu/nu male mice. We radiolabeled two anti-c-kit murine monoclonal antibodies (Mab1 and Mab2) with 125I using the chloramine-T or 3'-lodohippuryl-Ne-maleoyl-L-lysine (HML), and 111In using the 2-(p-isothiocyanatobenzyl) cyclohexyl-diethyl-enetriaminepentaacetic acid (CHX-A\"-DTPA). We performed cell binding, competitive inhibition and internalization assays in vitro, and biodistribution and SPECT imaging in tumor-bearing mice.\nResults: We isolated 18 c-kit stably expressing cell clones, and determined 6 of them highly express c-kit using western blotting analysis. Immunofluorescent staining of c-kit highly expressing cells showed that c-kit is primarily localized on the cell membrane like the original GIST cell line, and developed tumors in mice. 125I-MAb1, 125I-MAb1(HML), 125I-Mab2, 111In-MAb1 and 111In-MAb2 specifically bound to c-kit expressing cells with affinity constants of 4.3x108M-1, 5.2x108M-1, 3.5x108M-1, 3.4x108M-1 and 3.9x108M-1, respectively. These antibodies were internalized, and 111In-labeled Mab1 highly accumulated in xenografted tumors, which were visualized by SPECT.\nConclusion: These results showed that the radiolabeled c-kit antibody specifically accumulated in xenografted tumors and would be useful for the PET/SPECT imaging of GIST.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"Annual Congress of the European Association of Nuclear Medicine 2008","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sogawa, Chizuru"}],"nameIdentifiers":[{"nameIdentifier":"682309","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuji, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"682310","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sugyou, Aya"}],"nameIdentifiers":[{"nameIdentifier":"682311","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sudou, Hitomi"}],"nameIdentifiers":[{"nameIdentifier":"682312","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okada, Keiichirou"}],"nameIdentifiers":[{"nameIdentifier":"682313","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Arano, Yasushi"}],"nameIdentifiers":[{"nameIdentifier":"682314","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koizumi, Mitsuru"}],"nameIdentifiers":[{"nameIdentifier":"682315","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furukawa, Takako"}],"nameIdentifiers":[{"nameIdentifier":"682316","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Harada, Yoshinobu"}],"nameIdentifiers":[{"nameIdentifier":"682317","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saga, Tsuneo"}],"nameIdentifiers":[{"nameIdentifier":"682318","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"曽川 千鶴","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"682319","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 厚至","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"682320","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須尭 綾","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"682321","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須藤 仁美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"682322","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡田 圭一郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"682323","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"荒野 泰","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"682324","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小泉 満","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"682325","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古川 高子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"682326","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"原田 良信","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"682327","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀 恒夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"682328","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Development of anti c-kit monoclonal antibody probe for SPECT imaging of gastrointestinal stromal tumor","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of anti c-kit monoclonal antibody probe for SPECT imaging of gastrointestinal stromal tumor"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-10-21"},"publish_date":"2008-10-21","publish_status":"0","recid":"69526","relation_version_is_last":true,"title":["Development of anti c-kit monoclonal antibody probe for SPECT imaging of gastrointestinal stromal tumor"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:13:27.867247+00:00"}