{"created":"2023-05-15T14:50:46.876608+00:00","id":69415,"links":{},"metadata":{"_buckets":{"deposit":"ea60308c-f2a8-41a2-be3a-03906893dd3a"},"_deposit":{"created_by":1,"id":"69415","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"69415"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00069415","sets":["10:28"]},"author_link":["681240","681243","681247","681244","681246","681245","681241","681242","681248"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2008-07-16","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"MULTIMODAL QUANTUM DOTS: DIRECT INTRACELLULAR DELIVERY, PHOTOSENSITIZATION AND CYTOTOXIC EFFECTS\n\\nZhelev Zhivko,1 Aoki Ichio,1 Bakalova Rumiana,1 Gadjeva Veselina,2 Kanno Iwao1\n1. Department of Biophysics, Molecular Imaging Center, National Institute of Radiological Sciences (NIRS), 4-9-1 Anagawa, Chiba 263-8555, Japan\n2. Department of Chemistry and Biochemistry, Trakia University, Stara Zagora, Bulgaria\n\\nThe architecture of biocompatible nanoparticles (such as quantum dots, QDs) allows a development of novel molecular probes with dual- or multimodality for simultaneous application in different imaging techniques [e.g., optical imaging, magnetic resonance imaging (MRI), positron emission tomography (PET), X-ray imaging, etc.]. In the present study, we describe two multimodal QD probes with combined fluorescent and paramagnetic spectral characteristics and their intracellular delivery, photosensitization and cytotoxic effects. Using fluorescent confocal microscopy, we observed that the probes were characterized with easy and comparatively fast intracellular delivery in viable cells without transfection techniques that are inherently toxic or keep a risk of artifacts. Both probes penetrated through cytoplasmic membrane via endocytosis. The probes were localized into the cytoplasm, uniformly distributed. Spots of the probes in close proximity to nuclear membrane were detected, which indicates an interaction with intracellular compartments. Using MRI, we quantified the amount of multimodal QDs delivered into the cells or adsorbed on the cell surface. Two independent calibration curves were used, based on the relationship between the concentration of the probe and T1 (longitudinal relaxation time) or T2 (transverse relaxation time) values, respectively. It was found that approximately 58% of the probes were delivered into the cells and/or bound on the cell surface. The photosensitization and cytotoxicity were clarified on several cultured cell lines before and after laser or ultraviolet irradiation. No cytotoxicity or photosensitization was detected up to 500 nM concentration.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"the 5th International Symposium on Nanoscience & Nanotechnologies","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Zhelev, Zhivko"}],"nameIdentifiers":[{"nameIdentifier":"681240","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aoki, Ichio"}],"nameIdentifiers":[{"nameIdentifier":"681241","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Bakalova-Zheleva, Rumiana"}],"nameIdentifiers":[{"nameIdentifier":"681242","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kanno, Iwao"}],"nameIdentifiers":[{"nameIdentifier":"681243","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"681244","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｚｈｅｌｅｖ Ｚｈｉｖｋｏ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"681245","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"青木 伊知男","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"681246","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"バカロバ ルミアナ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"681247","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"菅野 巖","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"681248","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"MULTIMODAL QUANTUM DOTS: DIRECT INTRACELLULAR DELIVERY, PHOTOSENSITIZATION AND CYTOTOXIC EFFECTS","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"MULTIMODAL QUANTUM DOTS: DIRECT INTRACELLULAR DELIVERY, PHOTOSENSITIZATION AND CYTOTOXIC EFFECTS"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-07-29"},"publish_date":"2008-07-29","publish_status":"0","recid":"69415","relation_version_is_last":true,"title":["MULTIMODAL QUANTUM DOTS: DIRECT INTRACELLULAR DELIVERY, PHOTOSENSITIZATION AND CYTOTOXIC EFFECTS"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:14:46.254055+00:00"}