@misc{oai:repo.qst.go.jp:00069413,
 author = {Konishi, Teruaki and Ishikawa, Takahiro and Iso, Hiroyuki and Kodama, Kumiko and Yasuda, Nakahiro and Hamano, Tsuyoshi and Suya, Noriyoshi and Imaseki, Hitoshi and 小西 輝昭 and 石川 剛弘 and 磯 浩之 and 児玉 久美子 and 安田 仲宏 and 濱野 毅 and 酢屋 徳啓 and 今関 等},
 month = {Jul},
 note = {A microbeam irradiation system, SPICE (Single Particle Irradiation system to Cell), is under completion at the National Institute of Radiological Sciences (NIRS). We have improved the beam size, which is now approximately 5 micrometers diameter, and the cell targeting system, which can irradiated maximum of 400~500 cells per minute. Two types of cell dish were specially designed. One is Si3N4 plate (3mmx3mm area with 1 micrometer thickness) consisting of 7.5 mmx7.5 mm frame of 200 micrometer thickness, and the another is Mylar film stretched by pressing with a metal ring. These cell dishes can be set on the voice coil stage equipped on the cell targeting system, which also consist of a fluorescent microscope and a CCD camera for capturing the cell image. This microscope system captures all the image of dyed cell nuclei and computes the coordinates of the cell position according to the fluorescence and synchronizes this with a single particle irradiation system consisting of a scintillation counter and a beam deflector for irradiation. All the procedures can automatically be performed after setting some parameters, such as a preset number of protons. The first experiment was reported a visualization of phosphorylated histone protein, gamma-H2AX, which is known as a marker for DNA double strand breaks to confirm whether the targeted cell was accurately irradiated.[1] We also achieved a result showing the production of intracellular reactive oxygen species using DCF-DA as a fluorescent marker in irradiated CHO-K1 cells. Now we are focusing on low dose effects and how to obtain survival curves to measure hyper-radio sensitivity by irradiating CHO-K1 cell lines and its DNA repair deficient cell lines., 11th International Conference on Nuclear Microprobe Technology and Applications},
 title = {Biological studies using mammalian cell lines and the current status of the microbeam irradiation system, SPICE},
 year = {2008}
}