@misc{oai:repo.qst.go.jp:00069338,
 author = {Nakayama, Fumiaki and Hagiwara, Akiko and Kimura, Miho and Imamura, Toru and Akashi, Makoto and 中山 文明 and 萩原 亜紀子 and 明石 真言},
 month = {May},
 note = {Ionizing radiation at high dose causes skin damages and hair loss, and hair follicle apoptosis is one of the major prognostic factors.  However, it is difficult to examine the hair loss in small animals such as mice, because total body irradiation (TBI) causes their death with a very high dose.  In this study, we aimed to establish the in vivo assay system of radiation-induced apoptosis in plucking-induced anagen hair follicles and used this assay to evaluate preventive effects of FGF1.  A portion of the dorsal skin of seven-week-old male BALB/c mice harboring uniform telogen phase hair follicles was depilated for induction of anagen.  BrdU incorporation and PCNA staining confirmed that the follicle keratinocytes were markedly proliferating in the following anagen phase.  The mice received TBI with gamma-rays at doses ranging 8-16 Gy at anagen V 6 days after depilation.  TUNEL assay was performed on paraffin-embedded sections of the dorsal skin to evaluate apoptosis over time after irradiation.  Irradiation extremely increased TUNEL-positive cells in these follicles in a dose dependent manner 24 hours after irradiation, although they were few in the non-depilated skin in case of irradiation.  FGF1 was administered intraperitoneally at a dose of 10-100 microgram 24 hours before TBI.  As a result, FGF1 decreased the proportion of TUNEL-positive cells in the follicles after irradiation.  TBI at a dose of 8 Gy causes not only hematopoietic damage, but also intestinal damage, resulting in death of mice about a week postexposure.  Therefore, the induction of anagen by plucking hair is useful to evaluate growth factors for effects on the radiation skin damages and hair loss, because it enables us to analyze the radiation-induced apoptosis 24 hours after exposure., International Investigative Dermatology 2008},
 title = {Evaluation of Radiation-Induced Hair Follicle Apoptosis in Mice and the Preventive Effects of FGF1 Therein},
 year = {2008}
}