{"created":"2023-05-15T14:50:38.322675+00:00","id":69247,"links":{},"metadata":{"_buckets":{"deposit":"f05a11c5-f278-4e1a-b015-f675ad6dcac0"},"_deposit":{"created_by":1,"id":"69247","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"69247"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00069247","sets":["10:28"]},"author_link":["679505","679507","679506","679508","679509","679504"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2007-12-15","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Tumor necrosis factor alpha (TNFalpha) is one of mediators of apoptosis as well as inflammation and immunity, and is produced upon radiation exposure.  TNFalpha is a unique proinflammatory cytokine whose signaling pathways are linked to both anti- and pro-apoptotic responses in many cell types.  To better understand the role of TNFalpha after high dose radiation, we have knocked-down TNFalpha by siRNA and examined changes of apoptosis-related proteins in human Jurkat T leukemia cells.  Jurkat cells were transfected with TNFalpha-specific siRNA.  Both control and TNFalpha-siRNA transfected cells were pretreated with an inhibitor of MEK, p38 MAPK or PI3K, and were irradiated with X-ray at a dose of 10Gy.  DNA fragmentation analysis showed that transfection with TNFalpha- siRNA significantly blocked radiation-induced apoptosis.  Western blot analysis showed that radiation induced phosphorylation of both ERK and p38 MAPK, which was further enhanced in TNFalpha- siRNA transfected cells even in the presence of a specific inhibitor of MEK or p38 MAPK.  Phosphorylation of Akt was also induced in TNFalpha-siRNA transfected cells after irradiation.  Radiation induced Bax expression and caspase-3 activation in the control cells, and transfection with TNFalpha-siRNA suppressed caspase-3 activation and up-regulated expression of Bcl-2 and Bcl-xL, whereas further enhanced Bax expression was observed.  In conclusion, radiation induces apoptosis through activation of MEK/p38 MAPK and caspase/mitochondria pathways requiring TNFalpha.  Further studies on the mechanisms are in progress.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第30回日本分子生物学会年会･第80回日本生化学会大会合同大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sudo, Makoto"}],"nameIdentifiers":[{"nameIdentifier":"679504","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{"nameIdentifier":"679505","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Akashi, Makoto"}],"nameIdentifiers":[{"nameIdentifier":"679506","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須藤 誠","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"679507","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔 星","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"679508","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"明石 真言","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"679509","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Radiation induces apoptosis through endogenous Tumor Necrosis Factor alpha via activation of MEK/p38 MAPK and mitochondria/caspases pathways in Jurkat T leukemia cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Radiation induces apoptosis through endogenous Tumor Necrosis Factor alpha via activation of MEK/p38 MAPK and mitochondria/caspases pathways in Jurkat T leukemia cells"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-12-26"},"publish_date":"2007-12-26","publish_status":"0","recid":"69247","relation_version_is_last":true,"title":["Radiation induces apoptosis through endogenous Tumor Necrosis Factor alpha via activation of MEK/p38 MAPK and mitochondria/caspases pathways in Jurkat T leukemia cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:16:43.721567+00:00"}