{"created":"2023-05-15T14:50:28.814028+00:00","id":69034,"links":{},"metadata":{"_buckets":{"deposit":"c5e1dd6c-5ed5-40fa-bd85-05122bc91eec"},"_deposit":{"created_by":1,"id":"69034","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"69034"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00069034","sets":["10:28"]},"author_link":["677538","677531","677537","677535","677540","677539","677536","677534","677533","677532"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2007-07-12","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"TP53 is a tumor suppressor protein functioning as a transcription factor to regulate plenty of genes involved in physiological responses to DNA damages, such as apoptosis, cell cycle arrest, DNA repair, etc. When induction of various TP53-target genes in various organs of mouse following whole body exposure to ionizing radiation (IR) was examined, it was shown that, in a same organ, induction varies from gene to gene, and, for a same gene, induction varies from organ to organ. Selective regulation of TP53-target genes may be the basis for the multi-functional property of TP53. In this regards, it is noteworthy that some transcription factors, such as Sp1, GKLF, Ets1, and IRF-1, have been reported to play a role in TP53-dependent transcriptional activation. It may be hypothesized that variable transcription factors and cofactors participate in regulation of TP53-target genes depending on organs, their physiological conditions, radiation doses, dose-rates and so on. We here comprehensively investigated the factors involved in regulation of a TP53-target gene CDKN1A(p21) after irradiation. We first carried out an electrophoretic mobility shift assay to analyze DNA-protein interaction in the upstream regions of the CDKN1A gene using 115 probes covering the region of approximately 2.9 kb in length. A drastic change of the DNA-protein interaction was observed at -2193bp/-2185bp which is closely located to the TP53 recognition site at -2261bp. Interestingly alteration of the DNA-protein interaction at -1368bp/-1333bp and -1268bp/-1233bp was observed only after irradiation with IR in the dose range of 0.2-2 Gy. Next, we carried out a reporter gene analysis using variously deleted CDKN1A gene promoter linked to the luciferase-encoding sequence. It was found that deletion of the region -1398bp/-1119bp drastically diminished the induction of the CDKN1A gene promoter after IR in the dose range of 0.2-2 Gy. As this region contains the loci where alteration of the DNA-protein interaction occurred after IR in the dose range of 0.2-2 Gy, it was suggested that some transcription factors, inducible after IR of this dose range, bind DNA at the loci -1368bp/-1333bp and -1268bp/-1233bp to regulate the CDKN1A gene promoter in cooperation with TP53.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"13th International Congress of Radiation Research","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nenoi, Mitsuru"}],"nameIdentifiers":[{"nameIdentifier":"677531","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Daino, Kazuhiro"}],"nameIdentifiers":[{"nameIdentifier":"677532","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakajima, Tetsuo"}],"nameIdentifiers":[{"nameIdentifier":"677533","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Taki, Keiko"}],"nameIdentifiers":[{"nameIdentifier":"677534","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KAKIMOTO, AYANA"}],"nameIdentifiers":[{"nameIdentifier":"677535","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"根井 充","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"677536","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"臺野 和広","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"677537","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中島 徹夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"677538","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"瀧 景子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"677539","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柿本 彩七","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"677540","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Involvement of multiple factors in regulation of the CDKN1A gene promoter in response to ionizing radiation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Involvement of multiple factors in regulation of the CDKN1A gene promoter in response to ionizing radiation"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-07-18"},"publish_date":"2007-07-18","publish_status":"0","recid":"69034","relation_version_is_last":true,"title":["Involvement of multiple factors in regulation of the CDKN1A gene promoter in response to ionizing radiation"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:19:16.171656+00:00"}