@misc{oai:repo.qst.go.jp:00069028,
 author = {Nishimura, Yoshikazu and Takeda, Shino and Kakuta, Izuru and Kim, Heesun and Nyuui, Minako and Ikota, Nobuo and 西村 義一 and 武田 志乃 and 角田 出 and 乳井 美奈子 and 伊古田 暢夫},
 month = {Jul},
 note = {Lactoferrin is an iron-binding glycoprotein with a molecular weight of about 80,000. Lactoferrin was originally isolated bovine milk and found in excretions of mammals and is released from neutrophil granules during inflammation. Lactoferrin is considered a multifunctional protein. In this study, we observed the radioprotective effect of lactoferrin in mice following whole-body X-ray irradiation. (1) C3H/He mice were used in this experiment. The mice were separated tow groups One group (25 mice) were fed an AIN-93 diet, which contained 0.1 % w/w lactoferrin. The other group (25 mice) was fed an AIN-93 diet and water ad libitum. The mice were kept for 30 days on each diet, and were then exposed 6.8 Gy of whole-body irradiation. The survival rates of these mice were observed within 30 days after irradiation. The survival rate of lactoferrin-diet mice were about 23% higher than those of mice on a standard diet, and the rates dropped sharply to a plateau at day 10 after X-ray irradiation. The scavenging abilities of lactoferrin were evaluated by the ESR spin-trapping method. Lactoferrin is a radical scavenger of hydroxyl radicals and the observations suggested that radical trapping or scavenging might cause the biological response. (2) 52 of C3H/He mice were exposed 6.8 Gy of X-ray irradiation. After the irradiation, LF (4 mg) was administered 26 of mice intraperitonealy and survival rates of these mice were observed within 30 days. The survival rates of lactoferrin-treated mice and non-treated mice were 93 % and 50 %, respectively within 30 days after X-ray irradiation. The radio-protective effects of lactoferrin after X-ray irradiation in mice were investigated by using the micronucleated plychromatic erythrocyte test.  Radiation induced apoptosis in mice given a single intraperitoneal injection of lactoferrin after X-ray irradiation was observed by hematoxylin-eosin staining. Lactoferrin inhibited splenocytes apoptosis and damage on a bone marrow cell after X-ray irradiation. These results suggest that lactoferrin can be used as a drug of radiation protective agent., Radiation Research Society},
 title = {Radioprotection of lactoferrin in sub-lethally X-ray irradiated mice},
 year = {2007}
}