@misc{oai:repo.qst.go.jp:00069009,
 author = {小西, 輝昭 and 石川, 剛弘 and 磯, 浩之 and 安田, 仲宏 and 大熊, 俊介 and 児玉, 久美子 and 濱野, 毅 and 酢屋, 徳啓 and 今関, 等 and 小西 輝昭 and 石川 剛弘 and 安田 仲宏 and 大熊 俊介 and 児玉 久美子 and 濱野 毅 and 酢屋 徳啓 and 今関 等},
 month = {Jul},
 note = {A microbeam irradiation system, SPICE(Single Particle Irradiation system to Cell), is under completion at the National Institute of Radiological Sciences (NIRS). We have improved the beam size, which is now approximately 10um diameter, and the cell targeting system, which can irradiated maximum of 1.0x105 cells per hour. The cell dish was specially designed and the cells were cultured on Si3N4 plate (3mmx3mm area with 1um thickness) consisting of 7.5mmx7.5mm frame of 200um thickness. The cell dish is set on the voice coil stage equipped on the cell targeting system, which also consist of a fluorescent microscope and a CCD camera for capturing the cell image. This microscope system captures all the image of dyed cell nuclei in a 3x3mm area and computes the coordinated of the cell position according to the fluorescence and synchronizes this with a single particle irradiation system consisting of a scintillation counter and a beam deflector for irradiation. All the procedures can automatically be performed after setting some parameters, such as a preset number of protons. SPICE is still under reliability assessment. The first experiment performed was a visualization of phosphorylated histone protein, gamma-H2AX, which is known as a marker for DNA double strand breaks using immuno-staining technique, to confirm whether the targeted cell was accurately irradiated. Now we are focusing on low dose effects and how to obtain survival curves to measure hyper-radio sensitivity by irradiating human normal fibroblast and DNA double strand break repair deficient cell lines. We have also performed an experiment to cross-check our survival curves with those obtained using other irradiation techniques, such as broad-beam irradiation. Preliminary results will be presented., 13th International Congress of Radiation Research},
 title = {Biological studies using human cell lines and the current status of the microbeam irradiation system, SPICE},
 year = {2007}
}