{"created":"2023-05-15T14:50:21.681581+00:00","id":68871,"links":{},"metadata":{"_buckets":{"deposit":"760736d1-2094-4604-90bb-c4184339736b"},"_deposit":{"created_by":1,"id":"68871","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"68871"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00068871","sets":["10:28"]},"author_link":["675920","675921","675916","675919","675914","675915","675918","675923","675922","675917"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2007-03-15","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"背景と目的\n高線量放射線被ばくは腸管粘膜細胞の障害、腸死など重大な急性障害をもたらす。本研究は、ラット正常腸管細胞株IEC-6を用い、高麗人参薬効主成分でステロール由来のGinsenoside Rg1やRb1の高線量放射線誘導apoptosisへの影響を検討した。\n材料と方法\nIEC-6細胞は、5%FCS含有DMEM培地を用いて培養し、それぞれ5、10、20μMのRg1やRb1を単独あるいはMEK、p38、PI3Kキナーゼなどの阻害剤と混合処理し、高線量（5、10、20 Gy）γ線照射前後のRg1やRb1のapoptosisへの影響をHoechst染色法、Flow Cytometry法やWestern blot法にて検討した。\n結果と考察\n　高線量γ線照射前後のRg1投与はIEC-6細胞のapoptosisを濃度依存的に抑制した。Rg1はMEKやp38阻害剤との混合投与によりさらにapoptosisを抑制したが、PI3Kキナーゼ阻害剤との混合投与はapoptosisを増強させた。Rg1 はAktのリン酸化を促進する一方、MAPK経路のERKやp38のリン酸化を抑制した。また、Bax、caspase 3などの発現を抑制したが、Bcl-2発現には影響を与えなかった。Rb1はγ線照射誘導apoptosisを抑制しなかった。以上より、Rg1はp38、ERKなどMAPK経路の抑制やPI3K/Akt 経路の活性化によってラット腸管細胞の放射線誘導apoptosisを抑制したと考えられる。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第９６回　日本病理学会総会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"崔, 星"}],"nameIdentifiers":[{"nameIdentifier":"675914","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田村, 泰治"}],"nameIdentifiers":[{"nameIdentifier":"675915","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"坂口, 奈賀子"}],"nameIdentifiers":[{"nameIdentifier":"675916","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｍａｊｉｄｆａｒ, Ｆａｒｈａｔ"}],"nameIdentifiers":[{"nameIdentifier":"675917","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"明石, 真言"}],"nameIdentifiers":[{"nameIdentifier":"675918","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔 星","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"675919","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田村 泰治","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"675920","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"坂口 奈賀子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"675921","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｍａｊｉｄｆａｒ Ｆａｒｈａｔ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"675922","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"明石 真言","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"675923","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"ラット小腸上皮細胞株IEC-6におけるGinsenoside Rg1の放射線障害防護作用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ラット小腸上皮細胞株IEC-6におけるGinsenoside Rg1の放射線障害防護作用"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-03-16"},"publish_date":"2007-03-16","publish_status":"0","recid":"68871","relation_version_is_last":true,"title":["ラット小腸上皮細胞株IEC-6におけるGinsenoside Rg1の放射線障害防護作用"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:21:08.341963+00:00"}