{"created":"2023-05-15T14:50:18.565548+00:00","id":68800,"links":{},"metadata":{"_buckets":{"deposit":"5fd1ed91-3aac-43d1-9bfc-6bf6afa90904"},"_deposit":{"created_by":1,"id":"68800","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"68800"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00068800","sets":["10:28"]},"author_link":["675248","675250","675252","675253","675255","675249","675251","675244","675247","675245","675254","675246","675243"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2006-09-08","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Heat-shock protein 90 (Hsp90) は ubiquitous 分子シャペロンタンパク質で、様々なタンパク質の安定、活性化や細胞周期チェックポイント、シグナル伝達機構、転写の調節と関連している。我々はプラスミドによりIGF-IRを過剰発現させたHeLa細胞（HeLa-IGF-IR）を用いて、Hsp90 阻害剤（17AAG）による放射線増感効果を検討した。Hela 及びHeLa-IGF-IR 細胞を150nMの17-AAGで24時間前処理し放射線感受性をコロニー形成法によって評価した。またMTT法、Westernblot、さらにapoptosisに対して免疫蛍光法やFlowcytometryも行い、放射線感受性を調べた。HeLa-IGF-IR細胞はHeLa細胞と比較し有意に放射線抵抗性を示したが、150nMの17-AAGを加えることによって、HeLa-IGF-IR細胞の放射線抵抗性は失われた。HeLa-IGF-IR細胞では17-AAGを加えることで、放射線照射後のPARP発現量が減少、またアポトーシスも増加することも確認した。MTT assayにより、17-AAG付加後、HeLa-IGF-IR細胞はHeLa細胞より成長が抑制された。IGF-IRを過剰発現しているような腫瘍では、IGF-IRタンパクを安定化するHsp90 阻害剤を用いることにより、下流のPI3-K, MAPKによる生存シグナル伝達経路を阻害し、アポトーシスが増加することで、有効な放射線増感につながる可能性があり、臨床応用が期待される。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本放射線影響学会第４９回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"于, 冬"}],"nameIdentifiers":[{"nameIdentifier":"675243","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"野口, 実穂"}],"nameIdentifiers":[{"nameIdentifier":"675244","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"関根, 絵美子"}],"nameIdentifiers":[{"nameIdentifier":"675245","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"二宮, 康晴"}],"nameIdentifiers":[{"nameIdentifier":"675246","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森, 亮"}],"nameIdentifiers":[{"nameIdentifier":"675247","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安, 隆一"}],"nameIdentifiers":[{"nameIdentifier":"675248","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"その他"}],"nameIdentifiers":[{"nameIdentifier":"675249","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"于 冬","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"675250","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"野口 実穂","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"675251","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"関根 絵美子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"675252","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"二宮 康晴","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"675253","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森 亮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"675254","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"675255","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Hsp90 inhibitorを用いた放射線増感による抗腫瘍効果の研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Hsp90 inhibitorを用いた放射線増感による抗腫瘍効果の研究"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-11-29"},"publish_date":"2006-11-29","publish_status":"0","recid":"68800","relation_version_is_last":true,"title":["Hsp90 inhibitorを用いた放射線増感による抗腫瘍効果の研究"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:21:57.479716+00:00"}