@misc{oai:repo.qst.go.jp:00068769,
 author = {Fujimori, Akira and Suetomi, Katsutoshi and Kojima, Ayako and Hou, Yagun and Egusa, Ayako and Okayasu, Ryuichi and Takahashi, Sentaro and 藤森 亮 and 末冨 勝敏 and 小島 綾子 and 方 雅群 and 江草 文子 and 岡安 隆一 and 高橋 千太郎},
 month = {Nov},
 note = {Gene expression profiling of normal human fibroblasts irradiated with heavy ion particles by a novel analysis method
Fujimori, A., Suetomi,K., Kojima, A., Fang, Y-Q., Egusa, A., Takahashi, S., Okayasu, R.
HiCEP (High-coverage expression profiling) is a novel comprehensive analysis method which is based on DNA finger printing and PCR amplification. It enables to detect any altered gene expression among 60-70% of all the actually transcribed genes in any eukaryotic cells and tissues. We previously applied HiCEP to a primary culture of normal human fibroblasts and observed gene expression responding to X-ray at the very low dose (10 mGy). As the result of screening approximately 23,000 transcripts, we have identified a set of CXC chemokines (CXCL1, CXCL2, CXCL6 and CXCL8) up-regulated by the 10 mGy X-rays in the normal human fibroblasts (HFLIII) (Cancer Res 2005; 65: 10159-10163). Those genes have hardly been expected from the previous studies using the higher (>100 mGy) doses of radiation. Our observation indicated that different molecular mechanisms are involved in the response to ionizing radiation with different doses /dose rates, suggesting that different cellular responses could be induced by ionizing radiation with different LETs. Accelerated heavy ion particles (at high LET) provide promising effects for radiotherapy of certain types of malignancy, however, the molecular basis of its advantage to gamma rays is not fully understood.This time, we applied HiCEP to normal human fibroblasts (HFL III) irradiated with high-LET radiation generated in our institute. More than 40 genes were found to be up-regulated in the irradiated cells by 3 folds within 4 hrs post-irradiation of carbon ion at 2Gy (70 keV/micro m). Those included the DNA damage-inducible genes (CDKN1A, CyclinG, Gadd45a), and also some unexpected genes, both predicted and unpredicted from the current public databases., 53th Annual Meeting of the Radiation Research Society},
 title = {Gene expression profiling of HiMAC-irradiated normal human fibroblasts by HiCEP},
 year = {2006}
}