{"created":"2023-05-15T14:50:11.595712+00:00","id":68642,"links":{},"metadata":{"_buckets":{"deposit":"aec949c9-9a63-49d8-b5af-e92c72de7643"},"_deposit":{"created_by":1,"id":"68642","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"68642"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00068642","sets":["10:28"]},"author_link":["673668","673671","673670","673665","673664","673659","673663","673669","673667","673658","673661","673666","673660","673662"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2006-09-08","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"我々は、Rag2欠損、scid、およびRag2欠損scid二重変異マウスを用いて胸腺リンパ腫の発生に働くがん遺伝子Notch1の変異を調べ、Rag依存性不正V(D)J組換え、およびRag非依存性微小相同配列対合末端結合の２経路により変異することを報告した。今回、胸腺リンパ腫を早期に自然発生するAtm欠損マウスにおいてもRag2が関与するかを検討したので報告する。［材料および方法］Atm欠損(C3H)とRag2欠損 (BALB/c)マウスの交配によって得られた野生系統、Atm欠損、Rag2欠損およびRag2Atm二重欠損マウス、各群５０匹以上を非照射あるいは２Gyの放射線で照射し、胸腺リンパ腫の発生を調べた。サザン法およびゲノムPCRによりNotch1遺伝子の変異頻度および変異様式を解析した。［結果および考察］野生系統、Rag2欠損、Atm欠損、Rag2Atm二重欠損マウスにおける胸腺リンパ腫の発生頻度は、放射線照射によりそれぞれ自然頻度0%から0%、39%から41%、74%から82%、55%から67%と増加したが、いずれにおいても照射による増加に有意差は認められなかった。Rag2欠損マウスは自然および放射線誘発胸腺リンパ腫を好発した。Atm欠損マウスにおける放射線誘発胸腺リンパ腫の頻度はRag2Atm二重欠損マウスと同等であったが、カプランマイヤー法で比較すると、Atm欠損マウスの腫瘍発生はRag2Atm二重欠損マウスに比べ明らかに早かった。以上より、Atm欠損マウスの胸腺リンパ腫発生経路に、Rag依存性経路とRag非依存性経路が存在すると推定された。放射線誘発胸腺リンパ腫におけるNotch1遺伝子の変異頻度は、Rag2、Atm、およびRag2Atm二重欠損マウスでそれぞれ30%、56%、46%であり、Rag2欠損マウスで低い傾向があった．変異様式の解析により変異誘発におけるAtm遺伝子の関与を報告する．","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本放射線影響学会第４９回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石井, 洋子"}],"nameIdentifiers":[{"nameIdentifier":"673658","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻, 秀雄"}],"nameIdentifiers":[{"nameIdentifier":"673659","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"東, 智康"}],"nameIdentifiers":[{"nameIdentifier":"673660","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"巽, 紘一"}],"nameIdentifiers":[{"nameIdentifier":"673661","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古瀬, 健"}],"nameIdentifiers":[{"nameIdentifier":"673662","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"久保, ゑい子"}],"nameIdentifiers":[{"nameIdentifier":"673663","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"野田, 攸子"}],"nameIdentifiers":[{"nameIdentifier":"673664","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石井 洋子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"673665","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 秀雄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"673666","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"東 智康","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"673667","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"巽 紘一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"673668","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古瀬 健","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"673669","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"久保 ゑい子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"673670","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"野田 攸子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"673671","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Atm欠損マウスにおける胸腺リンパ腫の発生経路","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Atm欠損マウスにおける胸腺リンパ腫の発生経路"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-09-11"},"publish_date":"2006-09-11","publish_status":"0","recid":"68642","relation_version_is_last":true,"title":["Atm欠損マウスにおける胸腺リンパ腫の発生経路"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:23:50.502006+00:00"}