{"created":"2023-05-15T14:50:08.558805+00:00","id":68575,"links":{},"metadata":{"_buckets":{"deposit":"4efdd10d-dd37-4a8b-a042-8b7fcbea921e"},"_deposit":{"created_by":1,"id":"68575","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"68575"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00068575","sets":["10:28"]},"author_link":["673042","673040","673034","673037","673038","673035","673036","673039","673041","673032","673043","673033"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2005-11-17","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"我々は、ヒト大腸ガン由来の細胞株HCT116において、非相同末端結合に関わる一連の遺伝子群をジーンターゲッティング法により破壊し、その遺伝子の機能解析を試みてきた。XRCC4は、DNA ligase IVと複合体を形成し、DNA二重鎖切断において非相同末端連結反応経路の最終段階、DNAの末端結合に必須の因子である。我々は、XRCC4遺伝子を破壊した細胞を作製し、その株における細胞生物学的な解析を行っている。 今回我々は、XRCC4 cDNAのC末側にFLAGタグを融合させたXRCC4 FLAG cDNA発現ベクターを構築し、XRCC4-/-細胞株に導入し、安定に発現する細胞系を作製した。X線に対する感受性を調べたところ、親株であるHCT116株とXRCC4-/-株との中間程度に感受性が回復することが明らかとなった。現在さらに詳細な解析を進めているところである。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本放射線影響学会第48回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森, 雅彦"}],"nameIdentifiers":[{"nameIdentifier":"673032","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中村, 篤子"}],"nameIdentifiers":[{"nameIdentifier":"673033","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"野代, 勝子"}],"nameIdentifiers":[{"nameIdentifier":"673034","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩見, 尚子"}],"nameIdentifiers":[{"nameIdentifier":"673035","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻, 秀雄"}],"nameIdentifiers":[{"nameIdentifier":"673036","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩見, 忠博"}],"nameIdentifiers":[{"nameIdentifier":"673037","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森 雅彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"673038","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中村 篤子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"673039","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"野代 勝子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"673040","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩見 尚子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"673041","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 秀雄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"673042","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"塩見 忠博","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"673043","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"ヒト体細胞におけるXRCC4遺伝子の機能解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヒト体細胞におけるXRCC4遺伝子の機能解析"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-07-05"},"publish_date":"2006-07-05","publish_status":"0","recid":"68575","relation_version_is_last":true,"title":["ヒト体細胞におけるXRCC4遺伝子の機能解析"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:24:36.332438+00:00"}