{"created":"2023-05-15T14:50:07.451974+00:00","id":68551,"links":{},"metadata":{"_buckets":{"deposit":"ce458bb2-138e-4231-bafd-223c479352fa"},"_deposit":{"created_by":1,"id":"68551","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"68551"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00068551","sets":["10:28"]},"author_link":["672797","672805","672804","672803","672808","672798","672802","672799","672807","672801","672796","672800","672806","672795"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2004-11-27","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"DNA二重鎖切断の非相同末端結合(non-homologous end joininng)修復に関与している蛋白であるDNA ligase IVの遺伝子配列に特異的な21塩基の二重鎖RNA(siRNA)を哺乳動物初代培養細胞にトランスフェクションすることにより、細胞が放射線感受性を獲得するかどうか検討を行った。siRNAを導入した正常初代線維芽細胞(HFLIII細胞)では、siRNA未導入細胞に比べてDNA ligase IV のRNA及び蛋白レベルの低下が認められた。また、免疫染色法による細胞内DNA ligase IV発現レベルの測定においても同様の低下が認められた。PCC法とコロニー形成試験を用いX線照射後のsiRNA導入細胞とsiRNA未導入細胞について放射線感受性の検討を行った結果、siRNA導入細胞において染色体のフラグメント数の増加並びに、程度は小さいものの有意な細胞生存率の低下が認められた。また、X線照射後のsiRNA導入細胞においてDNA-PKcsのリン酸化の亢進が観察された。これらの結果は、DNA ligase IVのsiRNAを導入する事により、HFLIII細胞の放射線感受性が増加した事を示唆している。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本放射線影響学会第４７回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"末冨, 勝敏"}],"nameIdentifiers":[{"nameIdentifier":"672795","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安, 隆一"}],"nameIdentifiers":[{"nameIdentifier":"672796","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋, 千太郎"}],"nameIdentifiers":[{"nameIdentifier":"672797","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森, 亮"}],"nameIdentifiers":[{"nameIdentifier":"672798","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡田, 真希"}],"nameIdentifiers":[{"nameIdentifier":"672799","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡部, 篤史"}],"nameIdentifiers":[{"nameIdentifier":"672800","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"久保田, 善久"}],"nameIdentifiers":[{"nameIdentifier":"672801","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"末冨 勝敏","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"672802","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"672803","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋 千太郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"672804","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森 亮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"672805","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡田 真希","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"672806","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡部 篤史","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"672807","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"久保田 善久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"672808","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"ヒト正常細胞におけるRNA干渉によるDNA ligase IV発現抑制と放射線感受性の関係","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヒト正常細胞におけるRNA干渉によるDNA ligase IV発現抑制と放射線感受性の関係"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-06-28"},"publish_date":"2006-06-28","publish_status":"0","recid":"68551","relation_version_is_last":true,"title":["ヒト正常細胞におけるRNA干渉によるDNA ligase IV発現抑制と放射線感受性の関係"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:24:52.569690+00:00"}