{"created":"2023-05-15T14:50:00.801683+00:00","id":68420,"links":{},"metadata":{"_buckets":{"deposit":"b6ee39b7-9bc3-4c7a-96ec-2846fc8a3ee6"},"_deposit":{"created_by":1,"id":"68420","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"68420"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00068420","sets":["10:28"]},"author_link":["671652","671640","671642","671655","671644","671656","671651","671641","671653","671646","671647","671659","671658","671649","671648","671645","671643","671654","671650","671657"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2004-11-27","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"放射線の継世代影響はマウス等の実験動物では確認されているが、ヒトでは確認されていない。実験動物を用いた継世代影響解析の結果からヒトに外挿する手法や直接ヒトの継世代影響を測定する方法の開発が求められている。我々は、照射雄親と非照射雌親及びその交配で得た仔マウス等のＤＮＡ塩基配列を解読し、ＤＮＡ塩基配列の変化した量を直接測定する方法で、γ線照射マウスの継世代影響を解析した。\n　照射雄Ｃ３Ｈマウスと非照射雌Ｃ５７ＢＬマウスを交配して得た約８０匹の仔マウス各々からＤＮＡを抽出し、１５０カ所の平均４００ｂｐのＳＴＳマーカーを増幅してdirect sequencingでＤＮＡ塩基配列約５００万塩基を解読し、誘発突然変異の量を測定して線量効果関係を調べた。direct sequencing は、ＤＮＡ塩基配列を忠実に決定できること、マウスの系統間ではＤＮＡ塩基配列にＳＮＰが存在することから、γ線照射で生じた仔マウスの誘発突然変異が両親のどちら由来かを判定できる有利性がある。雄親マウスへの０，１，２，３Ｇｙ照射３週間後に雌マウスと交配した実験の解析では、５００万塩基当たり誘発突然変異数は０，１，１，８個（欠失７と塩基変化１）で、３Ｇｙでの誘発突然変異頻度は5.3 x 10-7/bp/Gy又は2.2 x 10-4/locus/Gyであった。この結果は、精子細胞への照射による継世代影響の線量依存性は直線的では無いことを示唆している。この方法は放射線で起こる塩基変化、フレームシフト、欠失等全ての突然変異を定量的に検出できるため、突然変異の特徴や頻度を正確に反映し高感度である。また、遺伝的にヘテロでＳＮＰ頻度の高いヒトの継世代影響解析に極めて有効と期待される。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本放射線影響学会第４７回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"呉, 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康"}],"nameIdentifiers":[{"nameIdentifier":"671648","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"根井, 充"}],"nameIdentifiers":[{"nameIdentifier":"671649","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"呉 健羽","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"671650","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"森明 充興","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"671651","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"本郷 悦子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"671652","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"東 智康","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"671653","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡本 正則","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"671654","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"河野 明広","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"671655","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山内 正剛","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"671656","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"入谷 理一郎","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"671657","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大町 康","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"671658","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"根井 充","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"671659","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Direct sequencingによる継世代影響の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Direct sequencingによる継世代影響の解析"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-06-13"},"publish_date":"2006-06-13","publish_status":"0","recid":"68420","relation_version_is_last":true,"title":["Direct sequencingによる継世代影響の解析"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:26:20.624716+00:00"}