@misc{oai:repo.qst.go.jp:00068071,
 author = {Onoda, Makoto and Katsube, Takanori and Onoda, Mami and Ueno, Megumi and Inano, Hiroshi and 小野田 眞 and 勝部 孝則 and 小野田 真美 and 上野 恵美 and 稲野 宏志},
 month = {Oct},
 note = {Objective: The aim of the present study was to investigate the expression and localization of iNOS in the rat mammary gland irradiated by X-rays.
Materials and Methods: Lactating rats were irradiated by X-rays (~20 Gy) 24 h prior to isolation of the mammary glands. Homogenates and frozen sections were prepared for immunoblot analysis and immunohistochemistry of iNOS, respectively. Also, the mammary glands cultured in vitro were irradiated and the immunoblot analysis was performed.
Results and Discussion: Expression of iNOS increased in the mammary glands of irradiated rats. The immunoreactive signal of iNOS was localized to the epithelium of alveoli of the irradiated glands. However, the induction of iNOS was inconclusive in the glands of non-parous rats with or without irradiation. On the other hand, expression of iNOS in cultured mammary glands with X-rays increased in a dose-dependent manner and a time-dependent manner. In addition, nitrite concentration in the culture exposed to X-rays enhanced as an increasing iNOS expression. These results suggest that iNOS protein is induced locally in rat mammary glands X-irradiated and in turn an excess amount of NO is produced in the glands, and that NO generated excessively in the mammary glands may correlate with mammary tumorigenesis induced by X-rays., 第78回日本生化学会},
 title = {Induction of inducible nitric oxide synthase (iNOS) by ionizing radiation in rat mammary glands},
 year = {2005}
}