@misc{oai:repo.qst.go.jp:00068012,
 author = {Nakamoto, Daisuke and Yamamoto, Nobuharu and Takagi, Ryo and Uzawa, Katsuhiro and Shibahara, Takahiko and Tanzawa, Hideki and Mizoe, Junetsu and Noma, Hiroyasu and 中本 大介 and 山本 信治 and 高木 亮 and 柴原 孝彦 and 溝江 純悦 and 野間 弘康},
 month = {Oct},
 note = {Identifying the free DNA from tumor cells would thus provide a very effective means of diagnosing early metastasis and assessung the outcome of therapy. Research on Loss of heterozygosity(LOH) in various head and neck tumors has shown evidence of tissue type specific microsatellite regions with LOH. Considering such localized LOH as fingerprints of cancer cells, we searched for tumorrelated LOH locations in the free circulating DNA. The subjects of this study were 10 head and neck cancer patients due to receive heavy particle radiotherapy at NIRS (5:malignant melanoma; 5:adenoid cystic carcinoma). Venous blood samples were taken from them to extract free DNA from the srum and normal DNA from the white blood cells. Ten microsatellite regions scattered on chromosomes 1, 6, 9, 12, 17, and 19 of thus obtained DNA were analyzed by the PCR-LOH method. The results confirmed the presence of LOH, at least one region in all patients. The most frequently detected LOH marker was D9S171(9p21 region), at 80% in malignant melanoma(4/5) and 75% in adenoid cystic carcinoma (3/4). Consequently, LOH is present at a high incidence in D9S171 (9p21) in both malignant melanoma and adenoid cystic carcinoma. This suggete that a specific cabcer suppressor gene involved in malignant melanoma and adenoid cystic carcinoma is present on the short arm of chromosome 9. This also indicates that tumorrelated LOH detection in free circulating DNA may assist in the early detection of latent metastatic cells., 第６回アジア口腔顎顔面外科学会/第４９回日本口腔外科学会総会},
 title = {Clinical study of free circulating DNA of the head and neck cancer},
 year = {2004}
}