{"created":"2023-05-15T14:49:35.370295+00:00","id":67900,"links":{},"metadata":{"_buckets":{"deposit":"f1ba2a9d-7084-4148-b294-02025432294e"},"_deposit":{"created_by":1,"id":"67900","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"67900"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00067900","sets":["10:28"]},"author_link":["666968","666962","666969","666960","666963","666964","666958","666965","666967","666966","666959","666961"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2004-10-01","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"51株のヒト癌由来培養細胞の放射線感受性を調べて、高感受性を示すヒト食道癌（ESCC）由来細胞株（KYSE190）を見つけた。その細胞では放射線照射後のDNA-PKcs蛋白質がリン酸化されなかった。DNA-PKcsのFATドメインにアミノ酸置換（I3434T)を伴う突然変異が見つかり、その突然変異はDNA-PKcsの二次構造に変化をもたらすことがCHAFAS解析から予想された。ウエスタン解析により、KYSE190 細胞は完全長のDNA-PKcsを産生していること、およびATMの発現が減少していることを確認した。以上の結果より、FATドメインでの二次構造の変化によりATM,　Ku70およびKu86とDNA-PKcsとの親和性が減少すること、あるいはATMの減少によりDNA二本鎖切断（DSBｓ）の誘発情報がDNA-PKcsにまで伝達されないことが原因でDNA-PKcsがリン酸化されず、その結果、非相同性末端結合修復（NHEJ）機構が働かなくなると考えられる。放射線照射後のChk1およびChk2蛋白質もATMによってリン酸化されるが、その経路はNHEJには依存しない。現在、KYSE190 細胞におけるATMの機能を調べる目的で、放射線照射後のChk1およびChk2蛋白質のリン酸化を調べており、それらの結果も合わせて報告する。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第６３回日本癌学会学術総会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"伴, 貞幸"}],"nameIdentifiers":[{"nameIdentifier":"666958","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"道川, 祐市"}],"nameIdentifiers":[{"nameIdentifier":"666959","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石川, 顕一"}],"nameIdentifiers":[{"nameIdentifier":"666960","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"相良, 雅史"}],"nameIdentifiers":[{"nameIdentifier":"666961","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"島田, 裕"}],"nameIdentifiers":[{"nameIdentifier":"666962","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"稲澤, 譲治"}],"nameIdentifiers":[{"nameIdentifier":"666963","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井, 高志"}],"nameIdentifiers":[{"nameIdentifier":"666964","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"伴 貞幸","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"666965","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"道川 祐市","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"666966","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石川 顕一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"666967","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"相良 雅史","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"666968","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井 高志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"666969","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"放射線高感受性を示すヒトESCC由来培養細胞におけるATM蛋白質発現の減少とDNA-PKcs蛋白質のリン酸化不全","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"放射線高感受性を示すヒトESCC由来培養細胞におけるATM蛋白質発現の減少とDNA-PKcs蛋白質のリン酸化不全"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2004-10-04"},"publish_date":"2004-10-04","publish_status":"0","recid":"67900","relation_version_is_last":true,"title":["放射線高感受性を示すヒトESCC由来培養細胞におけるATM蛋白質発現の減少とDNA-PKcs蛋白質のリン酸化不全"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:32:26.108103+00:00"}