{"created":"2023-05-15T14:49:23.511517+00:00","id":67636,"links":{},"metadata":{"_buckets":{"deposit":"df5635a2-e6e4-4ce9-aab5-c00fc5c4c2bb"},"_deposit":{"created_by":1,"id":"67636","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"67636"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00067636","sets":["10:28"]},"author_link":["664741","664737","664739","664742","664740","664738"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2002-10-17","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"サイクリンキナーゼインヒビターp21(164 aa)の発現量は、p53による転写制御、およびユビキチン化-プロテアソームによる分解機構、により調節されている。これまでp21 C-terminal truncation mutantにより、p21-ユビキチン化制御領域の解析を行い、C-terminal148-157 aa 領域が効率的なユビキチン化に必須であることを報告した。今回、148-157が制御領域であることの証明を行った。\n【方法】His-Myc tag p21 mutant を真核細胞に発現させ、Ni カラムで精製後、anti-p21, anti-myc, anti-ubiquitin antibodyによるWesternblot解析を行った。\n【結果】148-157 aaがユビキチン化の制御領域であるのか、あるいは領域内の154Lysがユビキチン化部位であるのか、を確認するために K154Rmutantを作成し、ユビキチン化の効率を検討したところ、K154Rはfull lengthと同等にユビキチン化した。また、148-157のみを欠落したdeletion mutantでは、1-147 fragmentと同様に、ユビキチン化は起こらなかった。\n【結論】p21の効率的なユビキチン化には、C-terminal 148-157(TDFYHSKRRL)領域が必須の制御領域となっている。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"第７５回　日本生化学会大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"福地, 邦彦"}],"nameIdentifiers":[{"nameIdentifier":"664737","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"萩原, 民雄"}],"nameIdentifiers":[{"nameIdentifier":"664738","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"市村, 幸子"}],"nameIdentifiers":[{"nameIdentifier":"664739","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"巽, 紘一"}],"nameIdentifiers":[{"nameIdentifier":"664740","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"沼田 幸子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"664741","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"巽 紘一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"664742","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"CDKインヒビターp21のユビキチン化制御領域の同定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"CDKインヒビターp21のユビキチン化制御領域の同定"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2003-11-14"},"publish_date":"2003-11-14","publish_status":"0","recid":"67636","relation_version_is_last":true,"title":["CDKインヒビターp21のユビキチン化制御領域の同定"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:35:27.206991+00:00"}