@misc{oai:repo.qst.go.jp:00067561,
 author = {Watanabe, Atsuya and Ikehira, Hiroo and Obata, Takayuki and 渡辺 淳也 and 池平 博夫 and 小畠 隆行},
 month = {Oct},
 note = {(Aim of Study)
Current study is to evaluate the relative glycosaminoglycan(GAG) concentration of reparative cartilage after autologous chondrocytes implantation(ACI) with Gd-DTPA-enhanced magnetic resonance(MR) imaging. This method is based on the theory that both GAG and Gd-DTPA are negatively charged, that is, Gd-DTPA will distribute in cartilage inversely to the concentration of GAG.
\n(Methods)
Nine knees of nine patients, on which ACI of the femoral condyle was performed, were studied 6 to 36 month postoperatively. MR imaging was performed with a 1.5T magnet before and 2 hours after intravenous administration of Gd-DTPA. R1 relaxation rate measurements were made with an inversion recovery sequence. Concentration of Gd-DTPA in the cartilage was calculated by the difference between R1 before and after administration of Gd-DTPA.
Second arthroscopy was performed 12 months postoperatively, and biopsies were taken from the center of reparative cartilage. The histology of reparative cartilage samples was compared with concentration of Gd-DTPA penetrated into the reparative cartilage.
\n(Results)
The concentration of Gd-DTPA in the reparative cartilage was higher than that in native cartilage, and there was significant difference (p<0.05). This represents that the concentration of GAG in reparative cartilage was lower than that in native cartilage.
Of reparative cartilages obtained from 8 cases, the proportion of hyaline cartilage in one case was only 20 %.
In latter case, most of the tissue filling the reparative cartilage was fibrocartilage, and the concentration of Gd-DTPA penetrated into the reparative cartilage was higher than that in the other cases.
\n(Conclusion)
The evalution of GAG concentration in the reparative cartilage was possible by using the Gd-DTPA-enhanced MR imaging. This method may be very useful for non-invasive evalution of the maturing prosess in transplanted cartilage and the long-term durabiliry of reparative cartilage., OARSI 2003 World Congress on Osteoarthritis},
 title = {Evaluation of Relative Glycosaminoglycan Concentration of Reparative Cartilage with Magnetic Resonance Imaging After Autologous Chondrocyte Implantation},
 year = {2003}
}