{"created":"2023-05-15T14:49:16.536728+00:00","id":67486,"links":{},"metadata":{"_buckets":{"deposit":"c1833257-83b7-4dc1-871b-5c187fa3cc2a"},"_deposit":{"created_by":1,"id":"67486","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"67486"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00067486","sets":["10:28"]},"author_link":["663374","663375","663379","663369","663377","663378","663376","663373","663382","663384","663370","663372","663368","663380","663381","663371","663367","663383"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2003-10-04","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"宇宙での長期滞在において、発がんは特に関心が高いが、高LET放射線による発がん研究のデータは、まだ十分に蓄積されたとは言いがたい。そこで本研究では、マウスＴ細胞白血病モデル（胸腺リンパ腫）を用い、重粒子線誘発胸腺リンパ腫とＸ線誘発胸腺リンパ腫の発生メカニズムの違いを明らかにすることを目的とした。\n　５週齢のB6C3F1（雌）マウスにに290MeV炭素線（LET60keV/mm）（1.6Gy）を１週間おきに４回分割照射後、飼育し発生した胸腺リンパ腫について解析した。発生した胸腺リンパ腫からDNAを調整しPCR法でゲノムワイドにゲノムヘテロ接合性 (LOH)検索を行った。\n　炭素線およびＸ線誘発胸腺リンパ腫は、発生率（68.4％、76.3％）、潜伏期間（216.8±8.4日、218.9±7.9日）、発生時におけるマウスの体重（22.2±0.14g、24.5±0.44g）では大きな差は見られなかったが、胸腺リンパ腫の重さは、炭素線では250.3±17.5mg、X線では486.1±36.0mgであり炭素線誘発胸腺リンパ腫の方が有為に小さいことが明らかになった(p<0.01)。FACS解析では、発生した胸腺リンパ腫は分化型で、CD3の発現、CD4、CD8の比率、TL2の発現において炭素線誘発胸腺リンパ腫とＸ線誘発胸腺リンパ腫の間に差はなかった。また、LOH解析の結果、Ｘ線誘発および炭素線誘発胸腺リンパ腫とも第4、11、19番染色体でLOHの頻度が高いが第12番染色体においてはＸ線誘発胸腺リンパ腫の方がLOHの頻度が高い点で異なっていた。以上の結果は、炭素線とＸ線では発生した胸腺リンパ腫のphenotypeは基本的に類似しているが、LOH高頻度領域の分布に差があることから、胸腺リンパ腫発生メカニズムに一部違いがあることが示唆された。","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本宇宙生物科学会第17回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"柿沼, 志津子"}],"nameIdentifiers":[{"nameIdentifier":"663367","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"久保, 歩"}],"nameIdentifiers":[{"nameIdentifier":"663368","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"甘崎, 佳子"}],"nameIdentifiers":[{"nameIdentifier":"663369","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"野島, 久美恵"}],"nameIdentifiers":[{"nameIdentifier":"663370","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"物部, 真奈美"}],"nameIdentifiers":[{"nameIdentifier":"663371","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"馬嶋, 秀行"}],"nameIdentifiers":[{"nameIdentifier":"663372","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今岡, 達彦"}],"nameIdentifiers":[{"nameIdentifier":"663373","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"西村, まゆみ"}],"nameIdentifiers":[{"nameIdentifier":"663374","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"島田, 義也"}],"nameIdentifiers":[{"nameIdentifier":"663375","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柿沼 志津子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"663376","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"久保 歩","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"663377","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"甘崎 佳子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"663378","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"野島 久美恵","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"663379","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"物部 真奈美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"663380","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"馬嶋 秀行","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"663381","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今岡 達彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"663382","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"西村 まゆみ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"663383","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"島田 義也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"663384","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"重粒子線誘発マウス胸腺リンパ腫のゲノムヘテロ接合性","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"重粒子線誘発マウス胸腺リンパ腫のゲノムヘテロ接合性"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2003-10-03"},"publish_date":"2003-10-03","publish_status":"0","recid":"67486","relation_version_is_last":true,"title":["重粒子線誘発マウス胸腺リンパ腫のゲノムヘテロ接合性"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T20:37:10.503799+00:00"}